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黑色素通过MT2受体降低了猪中单个卵子细胞的过度多精子症

Jing-Tao Sun^1,2, Jia-Hui Liu^1,2, Lu Zhao^1,2

¹Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, 150030, China.

Scientific reports

|October 4, 2024

概括

黑色素增强了猪卵细胞细胞质成熟和胚胎发育在体外成熟 (IVM). 这一过程涉及改善器官细胞分布和皮质颗粒功能,促进受精和胚胎细胞形成率.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

黑素在体外成熟 (IVM) 过程中已知具有抗氧化作用.
在猪卵细胞体外受精 (IVF) 后,黑激素对预防多精子症的影响机制尚未完全理解.
了解这些机制对于改善辅助生殖技术至关重要.

研究的目的:

为了研究黑激素对猪卵细胞细胞质成熟的作用.
确定黑激素受体在调解这些效应中的作用.
检查黑激素对多精子和随后的胚胎发育的影响.

主要方法:

在IVM期间,猪卵细胞补充了黑色素.
使用 Melatonin 抗剂 (Luzindole 和 4-P-PDOT) 来研究受体参与.
卵细胞成熟,器官分布 (戈尔吉器官,内质网膜,线粒体),基因表达,皮质颗粒分布和胚胎发育 (双胞胎和胚胎细胞率) 被评估.

主要成果:

黑色素通过增强正常的器官分布和调节相关基因表达,显著改善了细胞质成熟.
这些效应被黑激素抗剂阻断,表明受体介导作用.
通过促进正常的皮质颗粒分布,黑色素补充改善了IVF后的双胞胎和胚胎细胞形成率.

结论:

黑色素通过其受体增强猪卵细胞的细胞质成熟.
在IVM期间补充黑素可以提高受精效率和胚胎发育能力.
这些发现凸显了黑激素在优化猪体IVM和IVF协议方面的潜力.

关键词:

黑色素是什么黑色素是什么黑色素是什么卵细胞是卵细胞中的一个.有机器人有机器人猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪多种子性多种子性.

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