从L-arabinose中高效地生产D-xylitol的Aspergillus niger的工程工程
Marcel Rüllke1, Veronika Schönrock1, Kevin Schmitz1
1Fungal Biotechnology in Wood Science, Holzforschung München, TUM School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.
Microbial cell factories
|October 4, 2024
概括
这项研究设计了Aspergillus niger从L-arabinose中产生D-xylitol,这是一个可持续的替代甜味剂. 修改后的真菌有效地将农业废物转化为D-xylitol,提供了一种环保的生产方法.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- D-xylitol是一种具有健康益处的天然甜味剂,但目前通过化学化生产是不可持续的.
- 在纤维素材料中丰富的L-阿拉比诺斯是D-xylitol生产中未得到充分利用的原料.
- 菌的托代谢自然会将D-二和L-二转化为D-xylitol.
研究的目的:
- 从L-arabinose中设计一种微生物菌株,以高效地生产D-xylitol.
- 为了增强D-xylitol的积累和分泌在Aspergillus niger中.
- 探索农业废物流的利用,以实现可持续的甜味剂生产.
主要方法:
- 基因改造Aspergillus niger以阻断D-xylitol降解通路 (ΔxdhA, ΔsdhA, ΔxkiA). 这种基因改造的目的在于阻止D-xylitol降解通路的发生.
- 从Saccharomyces cerevisiae中引入一个构成性活跃的水糖素 (Fps1),用于D-xylitol分泌.
- 使用来自林氏纤维素来源的L-阿拉比诺斯来培养和优化工程菌株.
主要成果:
- 工程Aspergillus niger生产高达45%的D-xylitol从L-arabinose在培养超水生物中.
- 带有三重淘汰和引入的Fps1载体的菌株显示出最佳的D-xylitol积累和分泌.
- 改造的真菌证明了有效利用L-阿拉比诺斯,对透应激耐受性的影响最小.
结论:
- 纤维状真菌,特别是黑色阿斯伯吉路斯,可以通过工程来有效地从L-arabinose中生物合成D-xylitol.
- 这种方法可以利用富含阿拉比南的农业废物,如甜菜纸,用于可持续的甜味剂生产.
- 开发的方法为传统的D-xylitol制造提供了更环保和更具成本效益的替代方案.
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