通过液体染色学-质谱学使用充电式气溶检测方法对溶解有机物质进行联合定量和表征
Stacey L Felgate1, Elizabeth Jakobsson2, Andrea Balderrama Subieta1,2
1Department of Chemistry, Uppsala University, Uppsala 751 23, Sweden.
Journal of the American Society for Mass Spectrometry
|October 5, 2024
概括
了解溶解有机物 (DOM) 组成是生态系统健康的关键. 一种新的综合分析方法揭示了瑞典水域的DOM特征,显示碳含量一致,但电离和染色分数的季节性变化.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 生态系统科学 生态系统科学
背景情况:
- 溶解有机物 (DOM) 对生态系统功能和生物地化学循环至关重要.
- 它的复杂分子性质阻碍了单一方法的全面表征和量化.
- 目前的量化方法通常依赖于代理,导致与组成数据的差异.
研究的目的:
- 开发和应用一条综合分析管道,用于同时表征和量化固相可提取DOM (SPE-DOM).
- 为了研究不同季节的瑞典水体中SPE-DOM的组成变化.
- 为了更深入地了解DOM在水生生态系统中的作用.
主要方法:
- 一个集成的液体染色学 (LC) -二极管阵列探测器 (DAD) -充电的气溶探测器 (CAD) -质谱 (MS) 管道被开发出来.
- 该方法用于分析夏季和冬季采样的8个瑞典水体的SPE-DOM.
- 分析的重点是染色体和可电离分数,以及整体碳含量.
主要成果:
- 染色体SPE-DOM比例与SPE-DOM总度正相关,但显示季节性一致性.
- 电离性SPE-DOM在不同地点一致,但在夏季样本中比例较高.
- 在所有地点和季节中,DOM碳含量保持稳定,约为40%.
结论:
- 似乎SPE-DOM度是由异色色彩输入驱动的.
- 夏季样本显示出更易于电离,更少的染色体材料的相对贡献,可能来自本土或选择性丰富的土著来源.
- 集成的LC-DAD-CAD-MS方法提供了超出单一分析技术的增强组成洞察力.
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