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抗生素耐药性和生态风险消除在现场规模建造的湿地,由综合元基因组学和元转录组学揭示

Yanhui Zhao¹, Qian Zhao¹, Daoxing Liu²

¹School of Environmental Science & Engineering, Shandong Key Laboratory of Water Pollution Control and Resource Reuse, Shandong University, Qingdao 266237, PR China.

Journal of hazardous materials

|October 5, 2024

概括

建筑湿地通过在沉积物和植物中积累水中的抗生素耐药性基因 (ARG) 显著减少. 这项研究揭示了ARG动态,宿主和减少表达,为废水处理提供了洞察力.

科学领域:

环境微生物学环境微生物学
废水处理技术废水处理技术
抗微生物耐药性抗微生物耐药性

背景情况:

建筑湿地 (CWs) 显示出控制环境抗生素耐药性基因 (ARGs) 的前景.
在CW中ARG去除,持久性和表达的机制尚未完全理解.
调查ARG动态对于评估环境风险至关重要.

研究的目的:

系统地研究ARG分布,移动性,表达和宿主在现场规模的CW中.
将CW中的ARG配置文件与平行自然河流系统进行比较.
阐明CWs在缓解ARG风险中的作用.

主要方法:

使用了元基因组和元转录组方法.
分析了来自CW和自然河的水,沉积物和植物样本.
进行源追踪分析以确定ARG来源和宿主.

主要成果:

碳水化合物在废水中减少了72.28%的ARG含量.
主要是在CW沉积物和植物中积累的ARG.
蛋白质细菌被确定为关键的ARG宿主,ESKAPE病原体持续存在,但显示ARG表达减少.

结论:

碳水化合物有效地通过沉积在沉积物和植物中来减少ARG在水中的丰度和表达.
了解ARG主机动态和表达对于有效的CW设计至关重要.
这项研究提供了关于控制废水处理中的抗菌素耐药性的见解.

关键词:

其他AMR风险 AMR风险抗生素耐药性基因是抗生素耐药性的基因.建造的湿地建造的湿地大基因组和转录基因组.采购跟踪跟踪的方法

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