53BP1通过调节线粒体恒常状态来调节神经干细胞/原生细胞的自我更新能力
Yumi Sunatani1, Ryo Sakasai1, Tadashi Matsui1
1Department of Biochemistry I, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Biochemical and biophysical research communications
|October 5, 2024
概括
DNA修复因子53BP1通过调节活性氧物种 (ROS) 和线粒体功能来维持神经干细胞 (NSC) 的自我更新. 用N-乙-l-氨酸恢复ROS平衡,在53BP1缺乏细胞中挽救了NSC干细胞.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 维持神经干细胞/原生细胞 (NSC) 自更新对于大脑发育和修复至关重要.
- 细胞内活性氧物种 (ROS) 水平是NSC干性的关键调节者.
研究的目的:
- 为了研究53BP1的作用,一个DNA损伤反应因子,在维持NSC干性.
- 探索53BP1,ROS水平,线粒体平衡和NSC自我更新之间的关系.
主要方法:
- 在ReNcell VM人类NSC中,53BP1的耗尽.
- 评估NSC的自我更新,差异化和ROS水平.
- 用N-乙-l-氨酸治疗,这是一个极端清除剂.
- 研究与ATM和FOXO3a.a.的功能相互作用.
主要成果:
- 53BP1的耗尽减少了NSC的自我更新,减少了神经圈的大小,并增加了分化.
- 53BP1缺乏导致细胞ROS水平升高和线粒体异常.
- 在缺乏53BP1的NSC中,N-乙-l-氨酸治疗恢复了自我更新和正常化的ROS水平.
- 53BP1与ATM和FOXO3a合作,维持线粒体平衡和NSC干性.
结论:
- 53BP1对于维持神经干细胞自我更新至关重要.
- 53BP1通过调节线粒体平衡和细胞内ROS水平来调节NSC干度.
- 这些发现突出了53BP1作为保护神经干细胞功能的关键因素.
相关概念视频
01:19Multipotency of Hematopoietic Stem Cells
3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K
01:14Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:28Biosynthesis of Nucleic Acids
9
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
9