基于超稳定的层级多孔核酸的MOF及其用于酶固定和催化
Wenyi Chen1, Shuping Lin1, Jiawei Zheng1
1School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China.
Colloids and surfaces. B, Biointerfaces
|October 5, 2024
概括
分层多孔金属有机框架 (HP-MOFs) 通过使用瓜诺辛5'-单酸盐 (GMP) 作为配体进行合成. 这些HP-MOF增强了酶活性和稳定性,显示了工业应用的前景.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 酶固定增强恢复,可重复使用性和稳定性.
- 层次性多孔金属有机框架 (HP-MOF) 适用于酶固定.
- 开发具有多种联体的HP-MOF仍然是一个挑战.
研究的目的:
- 为了合成新的HP-MOF,使用瓜诺辛5欧米诺单酸盐 (GMP) 作为有机配体.
- 为了研究合成的HP-MOFs的形态和多孔性.
- 为了评估这些HP-MOF中的固定酶在糖解和化方面的性能.
主要方法:
- 模板化乳化方法用于HP-MOFs合成.
- 使用GMP作为有机连接体与各种金属离子 (Zn2+,Cu2+,Cd2+).
- 在HP-MOF上对Candida antarctica lipase B (CALB) 的固定.
主要成果:
- 成功制造了具有微,中,和巨孔结构的超稳定晶体HP-MOF.
- 根据金属离子,HP-MOF表现出不同的形态 (状,棒状,板状).
- 不动化的CALB显示出增强的酶活性和稳定性,达到> 60%重量%的二甲基甘油 (DAG) 和> 90%重量%的三甲基甘油 (TAG).
- 在糖解中,经过六个重复使用周期后,固定化的酶保持了82.32%的初始活性.
结论:
- GMP是一种可行的有机连接体,用于构建多种HP-MOF.
- 惠普-MOF为固定酶提供了更好的性能和稳定性.
- 这项工作扩大了HP-MOF和酶固定技术的应用范围.
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