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环境盐度对牛奶鱼对急性低温压力的反应有所区别

Naveen Ranasinghe¹, Yi-Ren Huang², Wan-Hua Wu²

¹Department of Life Sciences, College of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan; The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.

The Science of the total environment

|October 5, 2024

概括

海水预条件保护乳鱼 (Chanos chanos) 免受寒冷压力,通过减轻活性氧物种 (ROS) 信号,减少细胞死亡和水产养殖中的经济损失.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类生理学鱼类生理学
环境压力生物学

背景情况:

全球变暖加剧了极端寒冷事件,造成了水产养殖业的重大经济损失,特别是亚热带地区的奶鱼 (Chanos chanos).
乳鱼水产养殖面临着大量的死亡率由于寒风暴,尽管他们的盐分耐受性.
了解冷压力机制对于减轻水产养殖损失至关重要.

研究的目的:

研究乳鱼中冷压诱导的细胞死亡的分子和细胞机制.
确定盐度在调节寒冷应激反应中的作用.
确定策略,以防止牛奶鱼在寒冷压力引起的死亡率.

主要方法:

已建立的初级乳鱼肝细胞培养物用于18°C的冷应激实验.
在淡水 (FW) 和海水 (SW) 条件下分析了抗氧化酶 (超氧化脱酶,催化酶) 的mRNA水平.
在FW和SW暴露于寒冷压力的乳鱼肝脏组织上进行了转录基因分析.

主要成果:

寒冷压力降低了FW肝细胞中抗氧化剂基因表达的调节,增加了活性氧物种 (ROS) 和细胞死亡.
在SW中,触酶表达被上调,与FW相比,ROS水平较低.
转录组分析揭示了不同的基因表达模式,SW特异性基因与新陈代谢相关,FW特异性基因与寒冷压力下的细胞增殖相关.
细胞染色体相关的基因,一个主要的ROS来源,被冷压下调在SW.

结论:

海水的盐度抵消了牛奶鱼的冷压力诱导的ROS信号.
在海水中预先调节乳鱼是一种可行的和实用的策略,以防止寒冷压力引起的死亡率.
这项研究提供了关于水产养殖物种盐度依赖的寒冷应激弹性方面的见解.

关键词:

寒冷的压力是冷的压力.低温症是什么意思低温症牛奶鱼是一种牛奶鱼.度是指度是指度.氧化应激是一种氧化应激.主要肝细胞主要是肝细胞.盐分盐分盐分盐分盐分

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