组织负荷降低了VS55和VMP冷保护溶液的临界冷却和升温速度
Zonghu Han1, Lakshya Gangwar1, Bat-Erdene Namsrai2
1Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Cryobiology
|October 5, 2024
概括
研究人员发现,与自由溶液相比,组织内的临界冷却和升温率 (CCR和CWR) 降低了. 这一发现对于改善玻璃化和重新加热过程中的冷保护剂 (CPA) 疗效至关重要.
科学领域:
- 低温生物学 低温生物学
- 生物物理学的生物物理.
- 组织工程是组织工程.
背景情况:
- 临界冷却和升温速率 (CCR和CWR) 是使用冷保护剂 (CPA) 成功玻璃化的关键指标.
- 之前的研究表明,组织内的临界率降低了,但对于实际的CPA度,这些并未得到充分探索.
研究的目的:
- 重新评估CPA度对脏组织内的临界率的影响.
- 了解组织平衡如何影响CPA对玻璃化和再加热的疗效.
主要方法:
- 临界速率的热量计测量是在脏组织上进行的,该组织与VS55和VMP CPA尾酒的不同度平衡.
- 在自由CPA溶液和脏组织中的临界率之间进行了比较.
主要成果:
- 与自由溶液相比,脏组织的临界冷却速率 (CCR) 降低了多达5倍,临界变暖速率 (CWR) 降低了多达9倍.
- 这些减少在一系列CPA稀释中被注意到,表明了一致的效果.
结论:
- 组织平衡显著影响CPA测量的临界速率,降低了成功玻璃化所需的速率.
- 这些发现支持开发更缩的CPA配方,以实现更大的组织的玻璃化和重新加热,例如人体大小的脏.
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