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用脂质抗原设计的细菌幽灵作为克拉米迪亚流产的无辅助剂疫苗

Huaiyu Zhang¹, Wei Li², Yunhui Li³

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

International journal of pharmaceutics

|October 5, 2024

概括

携带克拉米迪亚堕胎抗原的工程细菌幽灵引发了强大的免疫反应和对小鼠感染的保护,为无助剂疫苗铺平了道路.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
疫苗学疫苗学疫苗学

背景情况:

细菌幽灵 (BGs) 是有前途的疫苗平台,因为它们的辅助性质和抗原递送效率.
针对特定抗原的工程BG对于开发有效疫苗至关重要.

研究的目的:

为了设计大肠杆菌 (大肠杆菌) 幽灵来显示克拉米迪亚堕胎 (C. abortus) 的保护性抗原.
在小鼠模型中评估这些修饰BG的免疫性和保护效果.

主要方法:

经过修改的大肠杆菌幽灵使用 lpp'-ompA 嵌合体来显示C. abortus的巨细胞感染活性强化剂 (MIP) 抗原.
评估抗原表达,脂化和转移到BG表面.
评估抗原呈现细胞 (APC) 的吸收和激活在体外和体内.
接种了免疫的小鼠,并评估了幽默和细胞免疫反应,以及对C. abortus感染的保护.

主要成果:

在BG表面成功表达和脂化了MIP融合蛋白.
重组大肠杆菌幽灵 (rECG-lpp'-MIP) 增强了APC的吸收和激活.
免疫接种诱导了强大的幽默和Th1偏倚的细胞免疫反应,没有辅助剂.
在免疫小鼠中观察到C. abortus感染的显著清除和子宫病理的减少.

结论:

脂化抗原修饰细菌幽灵代表了开发有效C. abortus疫苗的可行策略.
这种方法有可能成为一个通用的,无辅助剂的疫苗平台.

关键词:

一个细菌鬼魂.克拉米迪亚流产的流产.脂质蛋白是一种脂质蛋白.疫苗疫苗疫苗疫苗 -----------------------------------------------------------------------------------------------------------------------------------------------------------------------

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