毛细管电泳的方法用于定量确定其微粒形式的水化氨酸
Yu Ermolenko1, M Antonova1, A Polivanova1
1D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
Analytical biochemistry
|October 5, 2024
概括
毛细管区域电泳量化水化氨酸 (HA) 和其细胞药物递送系统. 这种方法可以在没有样本准备的情况下准确地确定HA衍生物和药物物质,从而增强了制药分析.
科学领域:
- 药品分析 药品分析
- 生物聚合物的化学化学
- 药物输送系统 药物输送系统
背景情况:
- 化氨酸 (HA) 菌根对于向药物输送至关重要.
- 准确量化HA衍生物和封装药物对于质量控制至关重要.
研究的目的:
- 开发一种通用毛细管区电泳 (CZE) 方法,用于量化原生和疏水的HA.
- 建立基于HA的小粒体内的药物物质的同时量化方法.
- 证明CZE用于分析药物菌配方的应用.
主要方法:
- 毛细管区域电泳 (CZE) 用于定量分析.
- 优化了原生HA,油基氨酸和纳夫他胺结合HA的方法.
- 进行了药物物质 (洛佩拉米德,利法布丁) 和微粒中的基氨酸的同时分析.
主要成果:
- 为各种HA衍生物和分子量开发了一种通用CZE方法.
- 分析灵敏度 (LOD) 在HA的11-40微克/毫升,在药物的0.4-0.6微克/毫升之间.
- 在oleyl-HA微粒中实现了药物的准确,无需样本准备的量化.
结论:
- CZE提供了一种多功能工具,用于量化原生和改性HA.
- 开发的方法使得药物和HA衍生物的同时量化能够在细胞系统中实现.
- 这种方法支持质量控制和基于HA的药物输送系统的开发.
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