在和介质中跟踪微生物运动,使用光谱诱导极化
Sina Saneiyan1, Nicolette Filippone2, Frederick Colwell3
1Department of Earth Sciences, Binghamton University, Binghamton, NY, USA.
Journal of environmental management
|October 6, 2024
概括
光谱诱导极化 (SIP) 有效地监测了地下环境中的微生物化学反应. 这种地质物理技术显示,与微生物向营养源的移动有着强烈的信号相关性.
科学领域:
- 地质物理学 地质物理学
- 环境微生物学 环境微生物学
- 地下科学 地下科学
背景情况:
- 地下环境中的致病微生物对人类健康构成风险.
- 目前使用直接采样的检测方法在空间时间分辨率上是有限的.
- 监测微生物传输途径对于早期检测生物危险威胁至关重要.
研究的目的:
- 评估光谱诱导极化 (SIP) 在监测微生物化学反应中的效率.
- 评估SIP信号与Sporosarcina pasteurii在和多孔介质中的运动之间的相关性.
主要方法:
- 使用一个装满了太华沙的垂直柱子,和了KCl溶液.
- 在底部引入了Sporosarcina pasteurii,向上向碳源迁移.
- 时间SIP测量在列的底部,中间和顶部收集.
主要成果:
- 随着时间的推移,SIP信号的真实 (σ') 和虚拟 (σ′′) 导电部分都增加了.
- 假想导电性 (σ′′) 信号的峰值与微生物上升运动相关.
- 在没有碳源或微生物注射的对照实验中,没有显著的SIP信号变化.
结论:
- 光谱诱导极化 (SIP) 是一种可行的地球物理技术,用于监测地下环境中的微生物化学反应.
- SIP信号与微生物运动有很强的相关性,为地下污染检测提供了一个有希望的工具.
更多相关视频
11:17Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
Published on: February 17, 2014
13.6K
04:07Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
Published on: February 23, 2024
1.2K
相关概念视频
Microbial Growth Measurement: Indirect Methods
2
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
2
Measuring Reaction Rates
24.8K
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical...
24.8K
