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在心脏和肝脏纤维化中增加PRELP表达促进了原蛋白的产生

Yuto Yamauchi¹, Hiroki Mieno¹, Haruna Suetsugu¹

¹Department of Disease Control, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.

Biochemical and biophysical research communications

|October 6, 2024

概括

富含プロ林和氨酸的富含白氨酸的重复蛋白 (PRELP) 在纤维组织中升高调节,并特别表达在类似冠状细胞的肌纤维细胞体中. PRELP促进原蛋白的产生,使其成为纤维化的潜在治疗标.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
病理学病理学病理学

背景情况:

纤维化涉及肌纤维细胞的过度细胞外基质生产.
肌纤维细胞在纤维状况下可以采用类似于冠状细胞的特性,例如心肌梗塞后心肌梗塞.
这些类似状细胞的肌纤维细胞的确切性质尚未完全理解.

研究的目的:

为了研究蛋白和氨酸丰富的终端白蛋白丰富的重复蛋白 (PRELP) 在纤维化中的作用.
为了确定PRELP表达是否特异于类似于冠状细胞的肌纤维细胞.
探索PRELP在原蛋白生产中的功能及其作为治疗点的潜力.

主要方法:

在纤维化的心脏和肝脏中对PRELP表达的定量分析.
在特定细胞群中使用免疫组织化学识别PRELP表达.
关于SOX9调控Prelp表达的研究.
在肌纤维细胞中Prelp敲击后对原蛋白生产的评估.

主要成果:

在纤维化和炎症的心脏和肝脏中,PRELP的表达是上调的.
普雷尔普在状细胞类肌纤维细胞中得到特定的表达.
SOX9 调节 Prelp 的表达.
敲击Prelp减少了心脏和肝脏肌纤维细胞中的原体表达.

结论:

PRELP是纤维组织中状细胞样肌纤维细胞的特定标记物.
PRELP促进了原蛋白的产生,有助于纤维化.
在治疗纤维性疾病方面,PRELP是一个新的治疗点.

关键词:

纤维化纤维化纤维化马什 (MASH) 是一个非常重要的产品.心肌梗塞的心脏病发作肌纤维母细胞细胞.普雷尔普是一个很大的问题.

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