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来自Tenacibaculum maritimum的外膜囊泡 (OMV) 作为一种潜在的疫苗,用于对抗鱼类强菌病

M Pilar Escribano¹, Miguel Balado¹, Beatriz Santos¹

¹Department of Microbiology and Parasitology, Aquatic One Health Research Center (iARCUS) and Faculty of Biology-CIBUS, University of Santiago de Compostela, Santiago de Compostela, Spain.

Fish & shellfish immunology

|October 6, 2024

概括

来自Tenacibaculum maritimum的外膜囊泡 (OMV) 提供了对鱼的海洋菌病的显著保护. 这种无助剂的OMV疫苗有效地刺激了先天性和适应性免疫反应,为新型水产养殖疫苗铺平了道路.

科学领域:

水产养殖免疫学水产养殖免疫学
疫苗学疫苗学疫苗学
细菌学细菌学是一门学科.

背景情况:

海洋杆菌 (Tenacibaculum maritimum) 引起海洋杆菌病,对水产养殖造成重大威胁.
外膜囊泡 (OMVs) 是性和细胞介导免疫的强有力的诱导剂.
OMV 生产是T. maritimum的一个关键毒性因素.

研究的目的:

为了评估一个无辅助剂的原始T. maritimum OMV (Tm-OMV) 疫苗 (SP9.1-OMVs) 在幼鱼的疗效.
评估鱼在接种T. maritimum.疫苗和挑战后的免疫反应.

主要方法:

年轻的鱼用SP9.1-OMVs接种了疫苗.
接种疫苗的鱼类通过浴暴露而受到T. maritimum的挑战.
免疫反应的测量是通过抗Tm抗体标位和关键免疫基因 (il-1β,il-8,il-22,pcna,c3,cd4-1,ifng2,cd8α) 的表达.

主要成果:

SP9.1-OMV免疫接种提供了70%的相对百分比存活率 (RPS) 对T. maritimum挑战.
接种疫苗的鱼类显示了抗Tm抗体标位的剂量依赖性增加.
早在挑战后4小时就观察到先天性和适应性免疫基因的快速诱导.

结论:

没有辅助剂的TM-OMV有效地保护鱼免受T. maritimum感染.
疫苗引起强大的先天性和适应性免疫反应.
这些发现支持开发用于水产养殖的基于OMV的新型疫苗.

关键词:

水产养殖疫苗的疫苗.免疫反应的免疫反应.外膜囊泡 (OMV) 是一种这种疾病叫做tenacibaculosis.这就是Tenacibaculum.

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