GCRV编码的circRNAcirc_20与BIP和PERK形成一个三元复合体,通过抑制PERK-eIF2α通路来延迟病毒复制
International journal of biological macromolecules
|October 6, 2024
概括
草的reoviruscirc_20通过与BIP和PERK相互作用来抑制病毒复制,降低PERK通路和内质网膜应激 (ERS) 的调节. 这种病毒circRNA为病毒与宿主相互作用提供了新的见解.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 宿主-病原体相互作用
背景情况:
- 病毒循环RNAs (circRNAs) 越来越多地被认为是它们在宿主病毒相互作用中的作用.
- 草重复病毒 (GCRV) 编码了许多circRNAs,之前建议circ_20调节病毒复制.
研究的目的:
- 阐明GCRV编码的circ_20对病毒复制的调节机制.
- 为了研究circ_20的功能背后的分子相互作用.
主要方法:
- 在GCRV感染细胞中验证circ_20表达.
- 对circ_20对细胞内膜网膜应激 (ERS),PERK通路和反应性氧物种 (ROS) 生产的影响进行分析.
- RNA拉下测试以确定circ_20与BIP和PERK的相互作用域.
- 涉及circ_20.20的删除突变体的功能测试.
主要成果:
- 证实circ_20在GCRV感染期间负面调节ERS,PERK通路和ROS产生.
- circ_20 通过与 BIP 和 PERK 形成三元复合体来抑制 PERK 途径,从而延迟了 GCRV 复制.
- 确定了circ_20 (51-102 nt,451-502 nt,和514-565 nt) 的特定区域对BIP和PERK相互作用至关重要.
结论:
- circ_20 作为一种病毒因子,调节宿主细胞通路以影响病毒复制.
- circ_20与BIP和PERK的相互作用对于其对GCRV扩散的抑制作用至关重要.
- 这些发现加深了对病毒circRNAs在病毒宿主动态中的重要性的理解.
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