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细胞蛋白减弱黑色素瘤恶性瘤,但保护黑色素瘤细胞免受铁灭

Zuquan Zou¹, Qingyao Yu², Yong Yang³

¹Department of Health, Beilun District Center for Disease Control and Prevention, Ningbo, Zhejiang 315899, P.R. China.

Molecular medicine reports

|October 7, 2024

概括

细胞球蛋白 (CYGB) 作为治疗黑色素瘤铁亡的生物标志物. 高CYGB表达赋予耐铁亡,而它的损失增加了灵敏度,帮助患者选择向治疗.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生化学

背景情况:

皮肤恶性黑色素瘤是一种具有治疗限制的侵袭性皮肤癌.
细胞球蛋白 (CYGB) 是一种富含黑色素细胞的全球蛋白,在黑色素瘤发育过程中经常被沉默.
目前的黑色素瘤疗法面临着副作用和疗效方面的挑战.

研究的目的:

调查细胞蛋白 (CYGB) 作为基于铁灭的黑色素瘤治疗的潜在生物标志物的作用.
确定CYGB表达水平与黑色素瘤细胞对铁灭诱导物的敏感性之间的关系.

主要方法:

使用的黑色素瘤细胞系 (B16F10,A375,G361) 具有不同的CYGB表达.
服用铁亡诱导剂 (RSL3,埃拉斯) 来评估细胞活力和增殖.
通过过度表达和敲击来操纵CYGB表达.
在小鼠体内进行异种移植研究.

主要成果:

缺乏CYGB的黑色素瘤细胞对铁灭诱导剂敏感,而高CYGB的黑色素瘤细胞则具有抗性.
黑色素瘤细胞中CYGB的过度表达导致了铁灭性耐药性,减少了增殖,并抑制了上皮细胞-介质细胞过渡 (EMT).
通过CYGB介导的耐药性涉及EMT的抑制,谷氨酸过氧化酶4的上调,以及降低可变铁池.

结论:

细胞球蛋白 (CYGB) 作为一种潜在的治疗生物标志物,用于识别可能从铁灭治疗中受益的黑色素瘤患者.
CYGB表达水平可以预测对RSL3和erastin等铁灭诱导物的反应.
准CYGB可能在黑色素瘤治疗中提供一种新的策略.

关键词:

细胞球蛋白细胞球蛋白.表皮细胞-介质细胞过渡.铁性化 (ferroptosis) 是一种谷氨过氧化酶4的作用黑色素瘤是一种黑色素瘤.指 E-box-binding homeobox 1 盒子绑定本地盒子 1 盒子绑定本地盒子

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