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通过实验性设计方法优化的纳米粒子:细胞毒性,抗菌性,抗增殖性和抗氧化活性

Fatma Şayan Poyraz¹, Gülşah Akbaş¹, Dilek Duranoğlu²

¹Department of Molecular Biology and Genetics, Faculty of Art and Sciences, Yildiz Technical University, Istanbul 34349, Turkey.

|October 7, 2024

概括

这项研究开发了加载酸的多分子 (乳酸-co-糖酸) 纳米颗粒 (SaNPs),以增强酸的溶解性和抗癌活性. SaNPs在乳腺癌细胞上表现出显著的细胞毒性和亡作用,而对正常细胞没有毒性.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
药理学药理学是指药理学的学科.

背景情况:

酸 (Sa) 是一种植物衍生的化合物,具有广泛的生物活性,但由于水溶性差而受到限制.
纳米粒子配方是防水分子的有效载体,增强了它们的生物可用性和治疗潜力.

研究的目的:

为了合成和优化含有三酸的多 (乳酸-co-甘油酸) 纳米粒子 (SaNPs).
在乳腺癌细胞系 (MCF-7) 中评估SaNPs的体外生物活动,包括细胞毒性,亡诱导,增殖抑制和抗氧化作用.

主要方法:

使用实验设计合成和优化加载酸的PLGA纳米粒子 (SaNPs).
在MCF-7细胞中评估了细胞毒性 (MTT试验),抗亡 (TUNEL试验),抗扩散 (PCNA试验) 和抗氧化活性 (SOD,catalase,GSH,MDA,caspase-3).
在SaNPs和自由的西纳皮酸之间进行了比较分析.

主要成果:

优化SaNP (170.6 ± 3.6 nm) 对MCF-7细胞表现出剂量依赖的细胞毒性,在MCF10A细胞中没有观察到毒性.
SaNP显著降低了PCNA水平,并在MCF-7细胞中诱导了细胞亡.
用SaNP治疗改变了氧化应激标志物,包括增加了MDA和GSH,并降低了SOD和酶水平,表明显著的生物活性.

结论:

开发的SaNP配方有效地提高了酸的可溶性和抗癌效果.
在纳米颗粒中封装的多化合物显示出癌症药物设计的前景.
SaNP 作为一个有价值的模型,用于制备其他疏水生物活性物质,用于治疗应用.

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