Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Tubulin State Determines Proteopathic Fate.

Cytoskeleton (Hoboken, N.J.)·2026
Same author

Modulation of Biomolecular Aggregate Morphology and Condensate Infectivity.

Biomolecules·2026
Same author

NANOG Proximity Proteomics Maps Neighborhood Hubs Linked to Mesenchymal Stem Cell Stemness and Chromatin Control.

Biomolecules·2026
Same author

Condensate State as Determinant of Amyloid Pathology in Neurodegeneration.

Biomolecules·2026
Same author

Label-Free Microfluidic Modulation Spectroscopy Monitors RNA Origami Structure and Stability.

Biosensors·2026
Same author

Tubulin transforms Tau and α-synuclein condensates from pathological to physiological.

Nature communications·2026

相关实验视频

Updated: May 11, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K

多价值蛋白-核酸相互作用通过组成梯度多重角光散射探测.

Josephine C Ferreon1, Natee Kongchan2, Phoebe S Tsoi1

  • 1Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, Texas 77030, United States.

ACS omega
|October 7, 2024
PubMed
概括
此摘要是机器生成的。

构成梯度多角度光散射 (CG-MALS) 准确量化了蛋白质与核酸的相互作用. 这种方法是有效的评估结合亲和力和固态度,即使是复杂的,重复的元素.

更多相关视频

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

17.8K
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
10:00

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

54.3K

相关实验视频

Last Updated: May 11, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K
Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
10:41

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

17.8K
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS
10:00

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering SEC-MALS

Published on: June 20, 2019

54.3K

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 生物物理学的生物物理.

背景情况:

  • 像TDP-43和CELF1这样的RNA结合蛋白 (RBPs) 与重复的核酸元素相互作用.
  • 确定这些相互作用的精确固体测量是具有挑战性的传统方法,如电泳运动转移试验 (EMSAs).

研究的目的:

  • 评估构成梯度多角度光散射 (CG-MALS) 的实用性,以量化RBPs与重复核酸的结合亲和力和固态度.
  • 评估CG-MALS涉及TDP-43和CELF1与各种DNA和RNA序列的相互作用.

主要方法:

  • 利用组合梯度多角度光散射 (CG-MALS) 来分析蛋白质与核酸的结合.
  • 研究了TDP-43的RNA识别动机与TG/GU-重复ssDNA/ssRNA之间的相互作用.
  • 研究了CELF1的RNA识别动机与 (TG/UGUU/GU) 重复或富含GU的元素 (GRE) 之间的相互作用.

主要成果:

  • CG-MALS数据显示了结合亲缘关系和稳基度,与结合较短核酸重复次数的蛋白质结合的既定范围一致.
  • 该方法为蛋白质与核酸相互作用提供了可靠的定量估计.

结论:

  • 构成梯度多角度光散射 (CG-MALS) 是量化蛋白质与核酸结合的一种强大技术.
  • 在涉及重复元素的复杂相互作用中,CG-MALS特别有价值,用于确定固体几何学,包括高比率 (>2).