从l-lysine获得1,6-hexamethylenediamine的新型生物生产,基于人工一碳延长周期
Kaixing Xiao1, Dan Wang1, Xuemei Liu1
1Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, P. R. China.
研究人员对大肠杆菌进行了*Escherichia coli*的工程,用于生物生产1,6-甲二胺 (HMD),这是一个关键的尼龙-66前体. 这种新的"智能网代谢工程"方法从l-lysine获得了213.5mg/L的创纪录产量.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 1,6-甲二胺 (HMD) 是尼龙-66合成的关键前体.
- 目前的HMD生产依赖于化石燃料,促使对可持续生物生产的研究.
- 现有的生物基HMD方法面临昂贵的原材料和低产量的挑战.
研究的目的:
- 从可再生资源中设计一种微生物菌株,以高效地生物生产HMD.
- 开发一种新的代谢途径,用于*Escherichia coli*中的HMD合成.
- 优化生物转化过程,以提高HMD产量和转化效率.
主要方法:
- 在大肠杆菌中使用特定酶 (l-lysine α-oxidase, LeuABCD, KivD, Vfl) 重建HMD合成途径.
- 引入额外的酶 (d-alanine aminotransferase,KdcA) 来增强级联活性和基质耐受性,称为"智能网代谢工程".
- 开发一个+1C生物转化多酶级联,用于l-lysine转化为HMD.
主要成果:
- 最初的WD20菌株产生了46.7 ± 2.0毫克/升的HMD.
- 使用"智能网代谢工程"的优化菌株WD21实现了显著更高的产量.
- 最后的发酵过程产生了213.5 ± 8.7 mg/L HMD,这是迄今为止l-lysine生物转化报告的最高产量.
- 达到高达65%的l-lysine转化为HMD.
结论:
- 开发的"智能网代谢工程"战略使得从l-lysine中高效生物生产HMD成为可能.
- 这项研究在HMD的可持续微生物合成方面取得了重大进展.
- 取得的产量和转换效率为生物基HMD生产的工业应用铺平了道路.
更多相关视频
12:02An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
Published on: November 2, 2016
09:57A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
Published on: December 23, 2016
相关概念视频
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
Amino Acid Biosynthetic Pathways
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Preparation of Amides
The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...
Preparation of 1° Amines: Azide Synthesis
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
Preparation of Alkynes: Alkylation Reaction
Alkylation of terminal alkynes with primary alkyl halides in the presence of a strong base like sodium amide is one of the common methods for the synthesis of longer carbon-chain alkynes. For example, treatment of 1-propyne with sodium amide followed by reaction with ethyl bromide yields 2-pentyne.
