用涂层的DNA纳米结构作为控制细胞中 lysosomal 功能的平台
Petra Elblová1,2, Mariia Lunova1,3, Skylar J W Henry4,5
1Department of Optical and Biophysical Systems, Institute of Physics of the Czech Academy of Sciences, Prague, 18221, Czech Republic.
Chemical engineering journal (Lausanne, Switzerland : 1996)
|October 7, 2024
概括
DNA纳米结构 (DNs) 提供了多功能工具,通过向 lysosomal 活动来调节细胞功能. 表面的修饰控制了溶酶体的pH值,影响了细胞代谢,免疫信号,甚至是生物医学应用的细胞死亡.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 细胞生物学 细胞生物学
背景情况:
- DNA纳米技术为生物医学应用提供了先进的工具.
- 用像DNA纳米结构 (DNs) 这样的纳米材料准 lysosomal 功能提供了一种系统的方法来控制细胞过程.
研究的目的:
- 开发一种基于DNA纳米结构的多功能平台,用于调节细胞功能.
- 研究DN的不同度和表面装饰如何影响 lysosomal活动和下游细胞反应.
主要方法:
- 用不同的 (含甲素和甲素) 对DNA纳米结构 (DNA) 的表面功能化.
- 对具有不同度的功能化DNA的细胞进行治疗.
- 评估溶酶体活性 (酸化/化),细胞形态,免疫信号 (STING激活),代谢活性和细胞毒性.
主要成果:
- 低度的脱素-DNA诱导了溶酶体酸化,改变了细胞代谢.
- 奥雷因-DNs促进了溶酶体化和STING激活.
- 高度的脱素-DNs导致溶酶体胀和形态变化而没有细胞死亡.
- 高度的黄素DNA导致了溶酶体破裂,线粒体损伤和显著的细胞毒性.
结论:
- 一个多功能DNA纳米结构平台可以通过准 lysosomal 活动,合理调节细胞功能.
- 表面装饰和DN的度依赖效应允许精确控制细胞结果.
- 这个平台显示了开发新型抗癌疗法的潜力,增强内体逃生系统,并为 lysosomal pH 调节创建纳米平台.
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