动物起源的动物性病原体 Clostridioides difficile 的比较基因组学,以了解其多样性
Kumaragurubaran Karthik1, Subbaiyan Anbazhagan2, Murugaiyan Latha Mala Priyadharshini3
1Veterinary College and Research Institute, Udumalpet, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051 India.
3 Biotech
|October 7, 2024
概括
这项研究表明,狗中的Clostridioides difficile菌株携带毒素和抗菌素耐药性基因. 临床上显著的ST11和多药耐药菌株在动物之间传播,突出显示了潜在的动物传染风险.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 困难菌 (Clostridioides difficile) 是一种影响动物和人类的动物病原体.
- 有限的基因组数据存在于动物C. difficile的毒素和抗菌素耐药性基因.
研究的目的:
- 进行全基因组测序和对比的基因组学,从狗中分离的C. difficile.
- 在动物C. difficile菌株中识别毒素和抗菌素耐药性基因.
- 分析C. difficile的基因组结构和演变.
主要方法:
- 15个来自狗的C. difficile分离物的全基因组测序.
- 对比基因组学和泛基因组分析.
- 多部位序列类型化 (MLST) 用于遗传学分析.
主要成果:
- 在狗隔离物中确定已知的MLST类 (I,II,IV,V) 和一个神秘类.
- 全球第二次在狗中报告了ST11和ST54.
- 在109个基因组中,发现29个具有所有四种毒素基因;22个没有.
- ST11基因组,特别是来自MLST类V的基因组,经常携带毒素基因.
- 确定了特定的抗微生物耐药性基因,在ST11.11中,四环素耐药性突出.
- 非毒性菌株也含有抗微生物耐药性基因.
- 泛体分析表明,C. difficile. 的泛体结构是开放的.
结论:
- 有临床意义的ST11菌株在动物中循环.
- 在动物群体中存在多种耐药性非毒性C. difficile菌株.
- 开放的体表明C. difficile的进化和适应能力.
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