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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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相关实验视频

Updated: Jun 11, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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查询数据独立获取LC-MS/MS的亲和性蛋白质组学.

David L Tabb1, Mohammed Hanzala Kaniyar1, Omar G Rosas Bringas1

  • 1European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, The Netherlands.

Journal of proteins and proteomics
|October 7, 2024
PubMed
概括
此摘要是机器生成的。

数据独立获取 (DIA) 与质谱学 (LC-MS/MS) 结合,为亲和性蛋白质组学提供了更好的蛋白质量化. 与数据依赖获取 (DDA) 相比,DIA工作流,特别是使用FragPipe时,可以增强蛋白质检测并减少变化.

关键词:
亲密关系丰富的丰富.生物信息学是一种生物信息学.共同免疫性沉的发生.数据独立的获取获取数据.没有标签的量化.

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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相关实验视频

Last Updated: Jun 11, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物化学 生物化学
  • 分析化学 分析化学

背景情况:

  • 数据独立获取 (DIA) LC-MS/MS是一种用于亲和蛋白质组学和共免疫沉 (co-IP) 的强大技术.
  • 与数据依赖获取 (DDA) 相比,减少DIA中的量化变异性可以改善特定蛋白质相互作用者的检测.

研究的目的:

  • 在不同仪器制造商和生物信息工作流程中评估DIA实验中的光谱库,蛋白质数量缺失和变化系数 (CV).
  • 为了比较DIA与DDA的性能,用于亲和蛋白质组学应用.

主要方法:

  • 从六项使用DDA和DIA实验的研究中对亲和蛋白质的质疑.
  • 对四个当代DIA生物信息工作流程的分析:FragPipe,DIA-NN,Spectronaut和MaxQuant.
  • 评估光谱库生成,蛋白质定量和CV值.

主要成果:

  • 直接从DIA实验中生成的光谱库是有效的,与DDA的同等仪器时间相比.
  • 当代软件可能在实验中难以量化模糊的信号,例如模拟下拉或IgG控制.
  • 谱卫队,DIA-NN和FragPipe展示了对蛋白质量定量的控制良好的CV值.
  • 在DIA中使用FragPipe进行光谱图书馆构建和量化,超过DDA,产生更多量化的蛋白质和较低的CVs.

结论:

  • DIA-LC-MS/MS是一种强大的亲和性蛋白质组学方法,在蛋白质量化和检测方面比DDA具有优势.
  • 优化的DIA生物信息工作流程,如FragPipe,可以显著提高蛋白质组分析的深度和可靠性.
  • 需要仔细的实验设计来管理控制实验中的模糊信号,以获得准确的蛋白质组概况.