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YTHDF3通过通过m6A甲基化调节FGF2来调节乳腺癌细胞的进展

R F Gong^1,2, Z H Zhang², T T Sun³

¹Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Frontiers in cell and developmental biology

|October 7, 2024

概括

YTHDF3通过m6A修饰来稳定FGF2表达,影响瘤进展并提供潜在的预后生物标志物,作为乳腺癌中的瘤基因.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

乳腺癌 (BC) 是女性癌症死亡的主要原因.
RNA N6-甲基氨酸 (m6A) 修饰是一种新兴的基因表达调节剂.
YTH N6-甲基氨酸RNA结合蛋白3 (YTHDF3) 与各种癌症有关,但其在BC中的作用需要澄清.

研究的目的:

研究YTHDF3在乳腺癌中的功能.
确定BC.中的YTHDF3的下游目标和分子机制.
评估YTHDF3作为乳腺癌的潜在预后生物标志物.

主要方法:

西部涂抹,RT-qPCR和IHC用于评估YTHDF3表达.
MeRIP-seq和RNA-seq用于识别YTHDF3目标基因.
在体外功能测试,CRISPR-Cas9和救援实验以验证YTHDF3的作用.

主要成果:

YTHDF3是BC的高风险因素,其表达升高与预后不佳相关.
YTHDF3通过在FGF2mRNA编码序列中的m6A识别来准纤维细胞生长因子2 (FGF2).
通过降低FGF2蛋白稳定性,YTHDF3敲击抑制BC细胞自我更新,迁移和入侵.

结论:

YTHDF3通过一种m6A依赖机制促进FGF2的表达,在BC中起到瘤基因的作用.
YTHDF3和FGF2在BC细胞恶性进展中发挥着关键作用.
一个基于YTHDF3的生物标志物面板可以帮助预测BC患者的预后.

关键词:

在FGF2上.这是一种N6-甲基氨酸.这就是YTHDF3的原因.乳房干器乳房干器表观遗传学是指表观遗传学.

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