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Updated: Jun 11, 2025

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高质量的基因组DNA提取协议用于细菌和菌物种

Yohannes Beyene Mekonnen1, Marina Elisabeth Aspholm1, Ephrem Debebe Zegeye1

  • 1Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway.

Current protocols
|October 7, 2024
PubMed
概括

这项研究详细介绍了一种成本效益高的DNA提取方法,用于Bacillus和Clostridium物种,产生高质量的基因组DNA,适合下一代测序 (NGS). 优化的协议确保了细菌菌株分析的可靠结果.

关键词:
细菌种类的细菌物种.克洛斯特里迪亚姆物种的克洛斯特里迪亚姆.提取DNA 提取DNA的方法在NGS质量方面,NGS质量.叶绿素-异氨基.

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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