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生物素度会影响在Corynebacterium glutamicum中的谷氨酸生产中起作用的厌食性反应

Takako Ochiai¹, Masaaki Wachi¹, Takashi Hirasawa¹

¹School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.

Microbiology (Reading, England)

|October 7, 2024

概括

生物素度影响了性酶 - - 酸核糖酶 (PC) 和酸酸核糖酶 (PEPC) - - 在Corynebacterium glutamicum中活跃,用于生产谷氨酸. 较低的生物素有利于PEPC,而较高的生物素允许PC和PEPC运作.

科学领域:

微生物学微生物学
生物化学生物化学
代谢工程是代谢工程.

背景情况:

谷氨酸菌 (Corynebacterium glutamicum) 是一种重要的工业微生物,用于氨基酸生产.
在C. glutamicum中,谷氨酸酸的产生受生物素的可用性和特定的代谢途径的影响.
由pyruvate carboxylase (PC) 和phosphenolpyruvate carboxylase (PEPC) 催化的纳普莱洛特反应对于为TCA循环提供中间体至关重要.

研究的目的:

研究生物素度对PC和PEPC在谷氨酸生产中的作用的影响.
为了确定Tween 40和青素诱导如何影响这些厌血路径.
阐明生物素在发酵过程中的PC和PEPC活性上的调节机制.

主要方法:

在C. glutamicum中利用了PC和PEPC的基因破坏剂.
在不同生物素度 (20微克/升和200微克/升) 下测量了谷氨酸产量.
在不同的诱导条件下,采用西式涂抹来评估与生物结合的PC水平.

主要成果:

在足够的生物素 (20μg/L) 处,PEPC主要驱动诱导谷氨酸生产的阿纳皮洛斯流.
随着生物素的增加 (200μg/L),PC和PEPC都会促进谷氨酸的产生.
40和青素降低了与生物素结合的PC在低生物素,但不是在高生物素度.

结论:

PC和PEPC都是C. glutamicum在谷氨酸生产过程中的功能性厌食酶.
生物素度是决定PC或PEPC优先使用的关键因素.
了解这种调节可以优化代谢工程策略,以提高氨基酸产量.

关键词:

在Corynebacterium glutamicum的研究中,我们发现过敏性反应的反应.生物生物是一种生物.谷氨酸酸是什么?谷氨酸酸是什么?基酸是氧化碳酶中的一种.皮鲁酸盐碳酸酶是什么

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