通过电子束诱导的黑色克拉姆 (L. Hepper) 突变体的全基因组测序进行突变概况
Sonali Vijay Habde1, Dhanasekar Punniyamoorthy1, Souframanien Jegadeesan1
1Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India.
International journal of radiation biology
|October 7, 2024
概括
电子束 (EB) 辐射有效地诱导黑色的全基因组突变,主要是单基替代和小. 这种突变谱造型有助于通过比较突变育种加速豆类作物的改进.
科学领域:
- 基因组学就是基因组学.
- 植物科学 植物科学
- 突变发生是突变发生的.
背景情况:
- 黑 (Vigna mungo) 是一种重要的豆类作物,具有重要的经济,营养和生态价值.
- 狭窄的遗传基因可以阻碍作物改进,需要诸如诱导突变发生等方法来产生新的变异.
研究的目的:
- 为了描述由电子束 (EB) 诱导的全基因组突变光谱,用黑色的图表.
- 为加速黑色基因基因改进提供对突变模式的洞察力.
主要方法:
- 用400 Gy电子束 (EB) 辐射黑色克拉姆基因型"Pant U-31".
- 使用Illumina和纳米孔技术重新测序一个稳定的突变 (PM-32,M6).
- 对父"Pant U-31"全基因组草稿组合的变异预测.
主要成果:
- 分析显示了728,161种变异,其中91.56%是单基替代 (SBS) (Ti:Tv比1.95).
- 印德尔构成了8.44%的变体,插入 (4.29%) 和删除 (多个基2-5bp) 是突出的.
- 在1271个基因中发现了2438种具有高/中等影响的基因变异,其中基因变异频率最高的基因是染色体Vm3.
结论:
- 电子束 (EB) 已被证实是诱导全基因组SBS和黑色色素中小的变异性工具.
- 对EB诱导的突变进行SNP级别的分析,有助于在豆类中进行比较突变育种研究.
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相关概念视频
In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
