上腺素和上腺素调节了Gallibacterium anatis中毒性因子的表达
Pablo A Rea Hernández1, Gerardo A Ramírez-Paz-Y-Puente1, Fernando Montes-García1
1Facultad de Estudios Superiores Iztacala, UNAM, Av. De Los Barrios 1, Los Reyes Iztacala, 54090, Tlalnepantla, Edo de México, Mexico.
Microbial pathogenesis
|October 7, 2024
概括
压力激素上腺素 (E) 和上腺素 (NE) 影响Gallibacterium anatis的毒性. 这些类甲基胺会影响细菌生长,粘合体表达和生物膜形成,这表明它们在家禽疾病的发病过程中起作用.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌病原体的产生
- 禽类的健康 禽类的健康
背景情况:
- 型细菌 (Gallibacterium anatis) 是一种机会性病原体,在中引起型细菌病.
- 主体压力,涉及诸如上腺素 (E) 和上腺素 (NE) 等类甲基胺,与G. anatis的致病性增加有关.
- 对于E和NE对G. anatis毒性因子的特定影响仍然在很大程度上是未知的.
研究的目的:
- 调查上腺素 (E) 和上腺素 (NE) 对两种Gallibacterium anatis菌株的生长,生物膜形成,粘合体表达和蛋白酶活性的影响.
- 为了确定QseBC双组件系统是否参与感知G. anatis.中的这些甲醇胺.
主要方法:
- 暴露G. anatis菌株 (溶血性12656-12和非溶血性F149T) 的不同度的E和NE.
- 测量细菌生长,生物膜形成和刚果红色结合.
- 蛋白酶活性和纤维素粘素的表达的分析.
- 通过PCR放大检测编码catecholamine受体的qseC基因.
主要成果:
- E和NE对两种G. anatis菌株的生长有不同的影响.
- 甲基荷胺提升了血液溶解菌株中的特定粘合素的调节,并在两种菌株中增加了蛋白质溶解活性.
- 在两种菌株中,E和NE减少了生物膜的形成,但增加了刚果红色的结合.
- 在两种G. anatis菌株中都发现了qseC基因,这表明了对catecholamines的潜在感应机制.
结论:
- 宿主衍生的类甲醇胺,上腺素和上腺素,可以调节Gallibacterium anatis的关键毒性因素.
- QseC受体的存在表明,G. anatis可以检测和响应宿主压力诱导的甲基荷兰胺水平的变化.
- 这些发现突出了一个潜在的机制,通过这种机制,宿主压力有助于G. anatis在中的致病性.
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