对内源和外源银纳米颗粒对微藻Chlorella vulgaris的影响的评估
Natalí Romero1,2, Adrianne Brito3, Horacio E Troiani4
1Laboratorio de Ecotoxicología, Facultad de Humanidades y Ciencias, Universidad Nacional del Litoral, Ciudad Universitaria, Paraje El Pozo s/n, Santa Fe, (CP 3000), Argentina.
World journal of microbiology & biotechnology
|October 7, 2024
概括
银纳米粒子 (AgNPs) 通过进入细胞和破坏结构来破坏水生藻类,如Chlorella vulgaris. 这导致透性增加,内部AgNP形成,最终导致细胞死亡,突出显示环境风险.
科学领域:
- 环境科学 环境科学
- 纳米技术 纳米技术
- 微生物学 微生物学
背景情况:
- 微藻是水生生态系统中至关重要的初级生产者.
- 银纳米粒子 (AgNPs) 的使用越来越多,引发了人们对其环境影响的担忧.
- 菌 (Chlorella vulgaris) 是一个全球重要的淡水藻类.
研究的目的:
- 研究银纳米粒子 (AgNP) 内化对微藻Chlorella vulgaris的影响.
- 阐明AgNP毒性和C. vulgaris.细胞损伤的机制.
主要方法:
- 对Chlorella vulgaris暴露于酸盐稳定的AgNP.
- 使用显微镜 (TEM) 分析AgNP积累和离子溶解.
- 细胞损伤的评估,包括器官结构,脂质含量和细胞活力.
主要成果:
- 在细胞壁中积累AgNP,增加透性,并允许AgNP和Ag+离子进入.
- 细胞内部的Ag+离子被转化为内源性AgNP,加剧了损伤.
- TEM揭示了叶绿体的失调,改变了细胞器官的功能,增加了细胞大小,导致细胞死亡.
结论:
- 在Chlorella vulgaris中,AgNP内部化会导致显著的细胞损伤和死亡.
- 外部和内部形成的AgNP都会导致毒性,可能是通过活性氧物种.
- 该研究强调了AgNP对广泛传播的水生藻类构成的生态风险.
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