优化,表征和生物安全性从乳清生产的胡卜素,使用微型白杆菌
Aml A Hegazy1, Samah H Abu-Hussien2, Neima K Elsenosy3
1Food Science Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt. aml_hegazy@agr.asu.edu.eg.
BMC biotechnology
|October 7, 2024
概括
优化Micrococcus luteus培养条件显著提高了胡卜素色素的生产. 这些提取物具有适度的抗微生物和抗氧化活性,具有证明的生物安全性,支持它们的潜在应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 自然产品 化学 化学
背景情况:
- 微球菌生产有价值的胡卜素颜料.
- 优化培养条件对于提高色素产量和表征生物活性至关重要.
- 了解抗微生物,抗氧化剂和细胞毒性质对于潜在的应用至关重要.
研究的目的:
- 通过使用统计媒体选,优化Micrococcus luteus (ATCC 9341) 的胡卜素颜料的生产.
- 描述产生的胡卜素的抗菌,抗氧化,细胞遗传和细胞毒性活动.
- 为了评估M. luteus胡卜素的生物安全性和潜在应用.
主要方法:
- 使用Box-Behnken设计来优化介质组件 (乳清度,注射剂大小,pH,温度,动速度).
- 高性能液体色谱 (HPLC) 用于胡卜素的鉴定.
- 通过抑制区直径 (IZD) 评估了抗菌活性.
- 使用DPPH试验 (IC50) 确定了抗氧化活性.
- 对正常小鼠肝细胞进行细胞毒性评估,并分析细胞遗传效应.
主要成果:
- 在优化条件下,可获得2.19 g/L的胡卜素,生产率为0.045 g L-1 h-1.
- 在HPLC分析中,确定了12种不同的胡卜素化合物.
- 提取物对格拉姆阳性细菌 (如Bacillus cereus,Staphylococcus aureus,E. faecalis) 具有中度的抗微生物有效性,但对格拉姆阴性细菌的活性有限.
- 抗氧化活性 (IC50) 为152.80毫克/100毫升.
- 细胞毒性低 (IC50 > 300μg/mL),没有发现显著的基因毒性作用.
结论:
- 统计优化显著提高了M. luteus.的胡卜素产量.
- 黄菌的胡卜素显示出有希望的适度抗微生物和抗氧化特性.
- 具有较低细胞毒性和生物安全性特征支持这些胡卜素的潜在应用.
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