相关实验视频
Updated: Jul 10, 2026

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
Published on: October 30, 2016
综合的多omics分析显示,ZZZ3/CD70轴是扩散大B细胞淋巴瘤细胞与自然杀手细胞相互作用的超强增强器驱动调节器
1Cancer Center, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
扩散性大B细胞淋巴瘤 (DLBCL) 细胞使用ZZZ3/CD70轴抑制自然杀手 (NK) 细胞活性. 通过PD-L1阻塞准这一轴可能提供一个新的DLBCL治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 瘤免疫微环境对于扩散性大B细胞淋巴瘤 (DLBCL) 病原发生至关重要.
- 连接超级增强剂 (SE) 与DLBCL细胞和免疫细胞相互作用的机制尚不清楚.
研究的目的:
- 研究SE调节基因在调节DLBCL细胞和瘤透免疫细胞之间的相互作用中的作用.
- 探索DLBCL中的ZZZ3/CD70轴及其作为治疗点的潜力.
主要方法:
- 对单细胞RNA-seq,大量RNA-seq和H3K27ac ChIP-seq数据的分析.
- 使用HOMER和Seurat进行生物信息分析.
- 在体外测试包括MTS和LDH释放测试,以评估细胞增殖和细胞毒性.
主要成果:
- DLBCL在B细胞和CD8+T细胞/NK细胞之间表现出增强的相互作用,特别是涉及CD70-CD27和MIF相关途径.
- NK细胞与DLBCL B细胞的相互作用最强.
- ZZZ3通过SE结合上调CD70转录;CD70沉默增强NK细胞增殖和DLBCL细胞死亡,ZZZ3过度表达可以逆转效应.
- 结合CD70沉默和PD-L1阻塞增加了DLBCL细胞死亡.
结论:
- DLBCL细胞利用ZZZ3/CD70轴抑制NK细胞增殖和细胞毒性活动.
- 针对ZZZ3/CD70轴与PD-L1阻塞一起,为DLBCL提供了一个潜在的治疗策略.
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