剪切诱导的循环逆转导致剪切薄化和自主自我愈合在可注射,保持形状的原凝中
Mahsa Jamadi Khiabani1, Sareh Soroushzadeh2, Ardeshir Talebi2
1Macromolecular Chemistry, Department of Chemistry─Ångström Laboratory, Uppsala University, Box 538, 751 21 Uppsala, Sweden.
ACS applied materials & interfaces
|October 8, 2024
概括
这项研究引入了一种新的基于原的可注射水凝,用于组织工程. 这种先进的生物材料提供了扩展的注射能力,自主自我愈合和稳定的形状保留,解决了该领域的关键局限性.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 可以注射的水凝对于组织工程中的细胞封装至关重要.
- 当前的水凝往往缺乏足够的注射时间窗口,自我愈合能力和形状稳定性.
- 细胞外基质 (ECM) 衍生水凝非常理想,但面临着挑战.
研究的目的:
- 开发一种可注射的,基于原的水凝,具有用于组织工程应用的增强性质.
- 克服当前注射水凝的局限性,特别是注射性,自我愈合性和形状保持性.
- 为了创建一个多功能生物材料用于体内应用.
主要方法:
- 开发一种以原为基础的水凝,通过 furan-maleimide 循环添加进行交联.
- 在延长时间窗口 (长达48小时) 中评估可注射性.
- 在注射后评估自主自我愈合特性.
- 在水性条件下测试形状和尺寸的保留.
- 使用原酶进行降解研究.
- 在细胞培养体内生物相容性测试.
- 在动物体内皮下植入大鼠以评估可吸收性和炎症.
主要成果:
- 开发的水凝可在制备后长达48小时内进行注射.
- 在注射后观察到水凝完全自主自我愈合.
- 这种水凝在缓冲中多年表现出极好的形状和尺寸保留.
- 在原酶治疗后几个小时内迅速降解.
- 通过体外细胞培养证实了生物相容性.
- 在未引起炎症的老鼠中,完全的in vivo可吸收性.
结论:
- 这种基于原的新型水凝成功满足了先进组织工程的关键标准.
- 这种生物材料为细胞封装提供了一个有前途的解决方案,可改善注射性,自我愈合性和稳定性.
- 水凝的生物相容性和可再吸收性使其适用于体内应用.
相关概念视频
01:15Drugs that Stabilize Microtubules
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
01:18Positive and Negative Feedback Loops
Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires maintaining an internal dynamic equilibrium:
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:06Multipotency and Niche of Bulge Stem Cell
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
01:23Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...