生物相容和安全的脱细胞化菜具有抗菌和伤口愈合活性
Rihab Ksouri1, Hamide Aksel1, Hamza Saghrouchni1
1Department of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Balcalı, Adana, Türkiye.
概括
研究人员开发了使用温和洗剂去细胞化菜叶的新方法. 由此产生的植物性支架显示出出色的生物相容性和抗菌性质,使其对组织工程和伤口愈合应用具有前景.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 植物性脚手架是一种植物性脚手架.
背景情况:
- 无细胞血管化结构对于组织工程至关重要.
- 使用苛刻洗剂的化学脱细胞化是常见的,但可能会损害组织.
- 植物组织是生物相容支架的潜在来源.
研究的目的:
- 为菜叶子开发基于低度洗剂的新型脱细胞化技术.
- 为组织工程应用创建一个非细胞植物矩阵.
- 评估衍生支架的生物相容性,伤口愈合潜力和抗菌活性.
主要方法:
- 使用Tween-20,二硫酸 (SDS) 和低度的甲酸 (SH) 来脱细胞化菜叶.
- 量化了DNA和蛋白质含量.
- 脱细胞化疗效通过血素和素 (H&E) 染色进行评估.
- 使用MTT和Scratch试验评估了生物相容性和纤维细胞伤口愈合.
- 测试了对黄金葡萄球菌的抗菌活性.
主要成果:
- 在1%的度下,在21°C和37°C之间,Tween-20产生了具有最佳形状,血管化和生物相容性的支架.
- 这些支架增强了细胞增殖和纤维细胞伤口愈合.
- 所有测试的脚手架类型都显示出对黄金葡萄球菌 (Staphylococcus aureus) 的显著抗菌活性,在4小时内达到100%的杀死率.
结论:
- 使用Tween-20的新型,温和的脱细胞化方法对于创建菜衍生的非细胞性支架是有效的.
- 这些植物性支架具有出色的生物相容性和强大的抗菌特性.
- 开发的支架适用于组织工程应用,并具有在伤口修复中药理学应用的潜力.
更多相关视频
相关概念视频
01:26Defense Mechanism Against Infection
Natural flora, body system defenses, and inflammation are natural barriers of the body against infectious agents regardless of previous exposure. Normal floras of the human body refer to the microbial population that colonizes the skin and mucous membranes.
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
01:18Surface Membrane Barriers
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:27Microorganisms in Agriculture and Food industry
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
01:27Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
01:23Microbial Spoilage of Food
Microbial food spoilage refers to the degradation of food quality resulting from the metabolic activity of microorganisms such as bacteria, yeasts, and molds. These microbes proliferate on various food substrates depending on factors such as moisture content, nutrient availability, and storage conditions, leading to undesirable sensory and structural changes.Bacteria are primary agents of spoilage in high-moisture, nutrient-dense foods like meat, milk, and vegetables. Microbial spoilage occurs...
01:27The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...