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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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相关实验视频

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Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
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实时可视化HIV-1RNA检测使用循环介导同热放大启用粒子扩散计.

Dong Hoon Lee1,2, Emeka Nwanochie2, Katherine N Clayton3

  • 1School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

ACS sensors
|October 8, 2024
PubMed
概括

这项研究引入了一种使用粒子扩散计 (PD) 来实时监测HIV-1RNA放大的新方法. 这种技术可以快速准确地量化病毒载量,显示出疾病管理的前景.

关键词:
土耳其人 RT-LAMP诊断 诊断 诊断 诊断 诊断扩散系数 扩散系数光是一种光.异热放大放大 异热放大放大粒子扩散度测试是一种粒子扩散度测试.实时实时的时间.

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Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
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科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断
  • 病毒学 病毒学

背景情况:

  • 像RT-LAMP这样的同热核酸放大测试 (NAAT) 可以快速实时检测病毒病原体.
  • 目前用于病毒载量监测的方法可能很复杂,需要专门的设备.
  • 需要强大的,可在现场部署的方法来准确量化病毒.

研究的目的:

  • 开发和验证一种使用RT-LAMP实时,半定量检测HIV-1RNA的新方法.
  • 在放大过程中利用粒子扩散计 (PD) 监测病毒度.
  • 评估开发的方法的速度,灵敏度和准确性.

主要方法:

  • 实时监测HIV-1RNART-LAMP使用新型粒子扩散计 (PD) 实现进行.
  • 在RT-LAMP反应期间,追踪了400nm光标记颗粒的扩散动态的变化.
  • 该方法在量化HIV-1RNA度的速度,灵敏度和准确性方面进行了测试.

主要成果:

  • 开发的基于PD的RT-LAMP方法在20分钟内实现了实时测量.
  • 检测HIV-1RNA度低至每μL的25个病毒颗粒被证明是.
  • 一项单盲研究显示,对于未知的HIV-1 RNA度,半定量检测具有10%的绝对误差.

结论:

  • 实时粒子扩散度读取是通过RT-LAMP量化HIV-1RNA的一个有希望的方法.
  • 这种方法有可能在艾滋病毒和其他慢性感染中快速和准确地监测病毒载量.
  • 该技术适用于现场使用和简单的仪器使其成为一种有价值的诊断工具.