5-oxoETE通过PLC/PKC/ERK通路触发NETs形成,从而促进抗脂综合征中的血栓形成
Xiaodong Song1,2, Xufeng Chen3, Dong Wang2
1Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
概括
增加的5-oxoETE水平导致中性粒细胞外细胞陷 (NET) 的形成和抗脂综合征 (APS) 中的血栓形成. 针对5-oxoETE或其受体可能提供新的APS疗法.
科学领域:
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
- 血管生物学 血管生物学
背景情况:
- 抗脂综合征 (APS) IgG通过中性粒细胞外细胞陷 (NET) 促进血栓形成.
- 连接APS IgG,NET和血栓形成的确切机制尚不清楚.
研究的目的:
- 为了确定血栓性APS患者的代谢变化.
- 阐明5-oxo-eicosatetraenoic acid (5-oxo-ETE) 在APS中NET形成和血栓形成中的作用.
主要方法:
- 在APS患者血清上的非向代谢量.
- 在体外中性粒细胞测试以评估5-oxo-ETE对NET和氧化应激的影响.
- 在APS小鼠模型中的体内研究使用5-oxo-ETE合成抑制剂或OXE-R抑制剂.
主要成果:
- 血清代谢结果显示,APS患者的5-oxo-ETE水平显著更高.
- 在体外,5-oxo-ETE因剂量而异,通过OXE-R和PLC/PKC/ERK信号传输增加了NETs的形成和氧化应激.
- 在体内,抑制5-oxo-ETE合成或OXE-R减少了APS小鼠的NETs形成和血栓形成.
结论:
- 5-oxo-ETE是APS中NET形成和血栓形成的关键媒介.
- 向5-oxo-ETE或其受体 (OXE-R) 是针对APS和其他NET相关的自身免疫疾病的潜在治疗策略.
相关概念视频
01:30Anticoagulant Drugs: Low-Molecular-Weight Heparins
636
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
636
01:16Clot Retraction and Fibrinolysis
4.7K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
4.7K
01:30Venous Thrombosis I: Introduction
3
Venous thrombosis, the most common disorder of the veins, involves the formation of a thrombus or blood clot associated with vein inflammation. It can be classified as either superficial vein thrombosis or deep vein thrombosis.Superficial Vein Thrombosis: This involves the formation of a thrombus in a superficial vein, usually the greater or lesser saphenous vein. Though less severe than deep vein thrombosis (DVT), SVT can lead to complications if untreated.Deep Vein Thrombosis (DVT): This...
3
01:20Extrinsic and Intrinsic Pathways of Hemostasis
6.3K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
6.3K
01:22Formation of the Platelet Plug
5.1K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
5.1K
01:24Disorders of Hemostasis
738
Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
738