通过涂层喷雾干燥提高抗菌效率的Polymyxin B的创新微封装
Amal Yousfan1, Arwa Omar Al Khatib2, Afrah M H Salman3,4
1School of Pharmacy, University of Reading, Reading RG6 6AD, U.K.
Molecular pharmaceutics
|October 8, 2024
概括
这项研究开发了一种新的微封装技术,用于使用多糖和喷雾干燥的Polymyxin B. 基于酸盐的微粒物显著增强了药物恢复,并表明持续释放和强大的抗菌活性对大肠杆菌和P. aeruginosa.
科学领域:
- 制药科学 制药科学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 聚米辛B是一种关键的抗生素,用于治疗多药耐药的格拉姆阴性细菌感染.
- 开发有效的药物输送系统对于提高Polymyxin B的治疗指数和克服耐药性至关重要.
- 微封装为控制药物释放和改善稳定性提供了一个有希望的策略.
研究的目的:
- 通过三流体喷嘴 (3FN) 喷雾干燥使用多糖化物开发和描述Polymyxin B的创新微封装方法.
- 评估不同多糖的影响 (基乙-环氧化,酸盐,奇托) 在Polymyxin B的回收,形态和释放动力学.
- 评估优化微粒素配方对抗格兰氏阴性细菌的抗菌疗效和抗菌膜潜力.
主要方法:
- 使用三流体喷嘴 (3FN) 喷雾干燥方法,用基乙与基乙-环氧,酸盐和酸盐微封装Polymyxin B.
- 使用高性能液态色谱 (HPLC) 来量化聚胺B的回收.
- 光标记,聚焦束反射率,里埃变换红外光谱 (FTIR) 和药物释放研究进行了表征.
- 抗菌活性 (MIC,MBC) 和抗菌膜测定针对*大肠杆菌*和*假菌菌*进行.
主要成果:
- 与酸盐 (ALGHCDHPLPM) 相比,含有高比的基和藻酸盐 (ALGHCDHPLPM) 的配方产生的Polymyxin B恢复率比基托增加了16倍.
- 微粒显示核心/外结构与一致的大小 (6.8 ± 1.2μm).
- 甲基酸盐配方表现出持续的药物释放 (26 ± 3%在24小时内) 和强大的杀菌作用对大肠杆菌和P. aeruginosa,具有显著的抗菌膜活性.
结论:
- 3FN喷雾干燥方法是有效的微封装Polymyxin B与多糖.
- 基于酸盐的微粒显示出优异的药物恢复,持续释放,以及增强的抗微生物和抗菌膜疗效.
- 这种先进的微封装技术对开发改进的抗微生物药物输送系统来对抗耐药细菌感染具有重大前景.
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