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在酵母中发现硫化感应E3酶的证据

Zane Johnson¹, Yun Wang¹, Benjamin M Sutter¹

¹Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA.

|October 8, 2024

概括

酵母硫氨基酸代谢是由Met4.4控制的. 该SCFMet30酶感知硫化和硫的可用性,调节Met4活动和基因表达.

科学领域:

* 分子生物学 * 分子生物学
* 生物化学 * 生物化学

背景情况:

*酵母中的硫氨基酸生物合成是由转录因子Met4.4调节的.
* SCFMet30 E3 泛基因酶通过泛基因化抑制硫丰度期间的 Met4 活性.
* Met30感知细胞硫状态的确切机制尚不清楚.

研究的目的:

*阐明F盒蛋白Met30感知硫的可用性的机制.
* 了解SCFMet30活动是如何调整以调节MET基因转录程序的.
* 通过Met30.30识别参与硫感应的分子组成部分.

主要方法:

*研究了转硫化途径在Met30调节中的作用.
* 在体内评估Met30对硫化的反应.
* 在Met30的WD-40重复区域中确定了关键的氨酸残留物,这对于硫感应至关重要.

主要成果:

* Met30 活性由通过转硫化通路的流量调节.
*硫化直接通过SCFMet30诱导Met4的无处不在化.
* Met30的WD-40重复中的特定的氨酸残留物对于检测细胞硫含量至关重要.

结论:

*SCFMet30通过转硫化途径动态感知硫代谢物流.
*这种传感机制允许精确调节氨酸和氨酸生物合成.
*这些发现揭示了酵母菌中硫平衡的新途径.

关键词:

E3 无处不在的合酶氨基酸氨基酸是一种氨基酸.代谢代谢代谢代谢营养素营养素营养素传感器传感器传感器传感器硫是一种硫.酵母酵母是一种酵母.

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