胰岛素的氧化和氧化性修饰改变了葡萄糖的摄取,细胞代谢和炎症分泌特征
Ramona Clemen1, Wiebke Dethloff1, Julia Berner2
1ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 17489, Greifswald, Germany.
Redox biology
|October 8, 2024
概括
使用气体等离子体生成的氧化胰岛素变体,对细胞代谢和葡萄糖吸收产生不同的影响. 这项研究强调了研究胰岛素中蛋白质氧化的重要性.
科学领域:
- 生物化学 生物化学
- 代谢调节 代谢调节 代谢调节
- 氧化压力是一种氧化压力.
背景情况:
- 胰岛素对葡萄糖,蛋白质和脂质代谢至关重要.
- 慢性高血糖会引起氧化应激,产生活性氧物种 (ROS),导致氧化胰岛素.
- 原生胰岛素与氧化胰岛素对葡萄糖平衡和细胞功能的生物学功能仍未得到充分研究.
研究的目的:
- 研究原生和氧化胰岛素对细胞代谢,增殖,葡萄糖转运体 (GLUT) 4,胰岛素受体 (INSR) 表达和葡萄糖吸收的影响.
- 探索气体等离子体中的不同反应性氧物种 (ROS) 组成如何改变胰岛素.
- 在氧化胰岛素变体 (oxIns) 中分析氧化后翻译修饰 (oxPTM) 模式.
主要方法:
- 利用气体等离子体方法来产生用于胰岛素氧化的多种ROS化合物.
- 采用高分辨率质谱测量来识别oxIns中的oxPTM模式.
- 评估了来自五种人体组织的细胞系上原生和oxIns的影响,测量了代谢活性,增殖,GLUT4和INSR表达和葡萄糖吸收.
- 评估了胰岛素氧化对胚胎血糖水平的 in ovo 影响.
主要成果:
- 原生胰岛素对不同的人类细胞系的代谢活性,增殖,GLUT4和INSR表达以及葡萄糖吸收产生异质影响.
- 特定的oxIns变体在研究的细胞系中显示出增强的细胞代谢和增殖.
- 与血排放线相关的胰岛素化与高葡萄糖摄取相关.
- 胰岛素氧化已被证明可以改变胚胎的血糖水平.
结论:
- 与原生胰岛素相比,氧化胰岛素变体具有不同的生物功能.
- 气体等离子体衍生的氧化和由此产生的oxPTMs显著影响胰岛素对细胞功能和葡萄糖平衡的作用.
- 对蛋白质氧化的进一步研究对于了解健康和疾病状态至关重要.
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