监管网络分析揭示了用甲基斯酸盐治疗的梨果中的基因代谢关系
Yubo Yuan1, Yangyang Chen1, Wanping Wu1
1Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Plant physiology and biochemistry : PPB
|October 8, 2024
概括
甲基斯酸盐 (MeJA) 治疗通过激活碳水化合物和氨基酸代谢来提高梨的质量. 这项研究揭示了参与这些代谢变化的关键基因和调节网络,为改善梨果质量提供了洞察力.
科学领域:
- 植物生理学 植物生理学
- 代谢学 代谢学 代谢学
- 分子生物学分子生物学
背景情况:
- 梨果的质量在经济上很重要,并受到成熟代谢物的影响.
- 甲基斯酸盐 (MeJA) 是一种已知影响植物代谢的植物激素.
- 关于MeJA对梨果质量途径的分子影响的了解有限.
研究的目的:
- 为了研究MeJA诱导的梨果代谢物MeJA诱导的变化的分子机制.
- 确定参与梨果质量改善的关键监管网络.
主要方法:
- 超高性能液体染色学Q精确轨道轨道质谱仪 (UHPLCQEMS) 用于代谢物分析.
- 全转录组测序以分析基因表达变化.
- 构建具有竞争力的内源RNA (ceRNA) 和miRNA-TF-target监管网络.
主要成果:
- MeJA治疗显著改变了梨果中的碳水化合物和氨基酸代谢.
- 确定了包括特定基因 (PEA,GAA,SDS),miRNAs (miR8033-x,miR4993-x),lncRNAs,circRNAs和转录因子 (TSO1) 在内的核心调节节点.
- 已建立的代谢物,基因和受MeJA影响的调节途径之间的关系.
结论:
- 阐明了MeJA在通过代谢途径调节来调节梨果的内部质量的作用.
- 揭示了MeJA对梨果的影响背后的分子机制.
- 为提高"Nanguo"梨的质量提供了理论基础.
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