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合成 lncRNA 位表现出 DNA 调节功能与序列演变

Gyan Ranjan¹, Vinod Scaria², Sridhar Sivasubbu²

¹CSIR Institute of Genomics and Integrative Biology, Mathura Road, Delhi 110024, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

|October 8, 2024

概括

合成长非编码RNAs (lncRNAs) 保持了跨物种的功能,尽管有序列分歧. 这些RNA位点通过cis-regulatory元素调节基因表达,有助于脊椎动物的进化.

科学领域:

基因组学就是基因组学.
进化生物学进化生物学
分子生物学分子生物学

背景情况:

合成长非编码RNAs (lncRNAs) 在物种中表现出低序列保护,这引发了关于它们的功能相关性的问题.
了解物种之间共享的lncRNAs的功能特征对于进化和发育研究至关重要.

研究的目的:

研究人类和斑马鱼之间的合成 lncRNAs 的功能特征.
探索这些lncRNAs在发育期间和不同细胞类型的基因调节中的作用.

主要方法:

在斑马鱼和人类胚胎干细胞 (H1-hESC) 中合成lncRNA表达模式的比较分析.
在合成 lncRNA loci.内识别和表征 cis调节元素和可转移元素 (TE).
检查lncRNA位点作为抑制剂,增强剂或转录起始点 (TSS) 的功能对蛋白质编码基因的检查.

主要成果:

合成 lncRNAs 在斑马鱼中表达高,主要位于蛋白质编码基因附近.
在早期发育 (斑马鱼) 和H1-hESC中,lncRNA位点与cis-regulatory抑制特征丰富,影响与发育相关的基因.
在以后的发育阶段和人类细胞系中,这些位点作为增强剂或TSS对蛋白质编码基因起作用.
人类合成的lncRNAs富含简单的重复元素,而斑马鱼的对应物则显示出LTR元素的丰富,这表明由cis调节功能驱动的序列进化.

结论:

合成lncRNA位点在脊椎动物物种中具有保留的功能,通过DNA元素介导,尽管有显著的序列分歧.
在lncRNA位点内TE含量的进化变化可能有助于新的cis-regulatory功能和脊椎动物创新.
这项研究强调了功能性保护对lncRNAs在进化中的序列保护的重要性.

关键词:

监管部门的监管部门.增强器增强器增强器可转移的要素是可以转移的.斑马鱼是一种斑马鱼.在ncRNA中,我们可以

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