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高准确度的DNA结合可以防止在酵母基因组中插入单个基

Jessica S Williams¹, Scott A Lujan¹, Mercedes E Arana¹

¹Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services, 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA.

Nature communications

|October 8, 2024

概括

在DNA复制过程中,DNA酶1 (LIG1) 的忠实性对于防止全基因组突变至关重要. 失去LIG1高保真性结合,特别是与受损的不匹配修复 (MMR) 导致特定的单基插入.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

在Okazaki碎片成熟 (OFM) 过程中,DNA复制需要DNA结合酶1 (LIG1) 来密封口.
以前的研究表明,LIG1结位的突变增加了单基插入.

研究的目的:

研究LIG1忠实性损失对DNA复制突变发生的全基因组影响.
确定LIG1突变和不匹配修复 (MMR) 缺陷对突变发生的协同效应.
识别与LIG1依赖插入相关的序列上下文.

主要方法:

在Saccharomyces cerevisiae中利用了一个突变的记者系统.
在野生型和突变LIG1菌株中分析了全基因组突变率.
评估了LIG1突变和MMR缺乏的综合影响.
描述了观察到的插入的序列特异性.

主要成果:

LIG1突变显著增加了整个核基因组的单基体插入.
在没有功能性MMR的情况下,插入率以协同作用的方式增加.
插入显示出强烈的序列上下文偏好,有利于将dGTP或dTTP纳入单核酸运行.

结论:

通过LIG1进行高保真性DNA结合,对于在滞后链合成过程中防止突变发生至关重要.
MMR与LIG1协同工作,以纠正特定的基础插入.
改变LIG1的突变性结合会产生独特的突变动机,强调精确的DNA修复的重要性.

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