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细菌性感染中与损伤相关的分子模式,包括与细菌DNA进行比较分析,是一种与病原体相关的分子模式

Helena Alpkvist^1,2, Ingrid Ziegler³, Paula Mölling⁴

¹Department of Medicine Huddinge, Unit of Infectious Diseases, Karolinska Institutet, Stockholm, Sweden.

Scientific reports

|October 8, 2024

概括

核DNA (nDNA) 作为败血症生物标志物表现有前途,在检测败血症和预测负面结果方面超过其他损伤相关分子模式 (DAMPs). 升高的nDNA水平表明败血症的严重程度,并且在结果不佳的患者中持续存在.

科学领域:

败血症的发病原因和生物标志物的发现.
传染病免疫学传染病免疫学
临床诊断临床诊断临床诊断

背景情况:

败血症是由损伤相关的分子模式 (DAMPs) 和病原体相关的分子模式 (PAMPs) 引发的,但同时研究很少.
了解细菌性感染中的DAMP动态对于评估疾病严重程度和患者结果至关重要.

研究的目的:

在患有细菌性感染的患者中研究四种DAMP (nDNA,mtDNA,HSP90α,HMGB1) 的动态.
将DAMP的诊断和预后能力与PAMP (16S rDNA) 和其他生物标志物进行比较.
为了将DAMP水平与败血症严重程度和患者结果相关联.

主要方法:

对83名患有社区发病细菌性感染的成年患者进行前性研究 (Streptococcus pneumoniae,Staphylococcus aureus,Escherichia coli).
在多个医院日内对nDNA,mtDNA,HSP90α和HMGB1的血液样本进行分析.
与16S rDNA,CRP,IL-6,IL-8和IL-10对抗DAMP的性能进行比较,用于败血症和负结果预测.

主要成果:

与其他DAMP和生物标志物相比,核DNA (nDNA) 显示出更高的诊断准确性 (败血症的ROC曲线为0.78,负结果为0.76).
两种nDNA和16SrDNA的高日1-2度独立地与败血症有关.
1-2天的高nDNA水平与负面结果独立相关,并且在预后不佳的患者中持续存在.

结论:

核DNA (nDNA) 是一种有前途的生物标志物,用于毒症检测和预测结果,其性能优于其他DAMP.
两种nDNA (DAMP) 和16SrDNA (PAMP) 独立地与败血症相关,nDNA也预测了负面结果.
在负面结果的患者中,nDNA的持续升高突显了它在败血症发病过程中的作用和临床效用.

关键词:

细菌血症是什么意思细菌性感染细菌性感染垃圾填埋场是一个垃圾填埋场.与损伤相关的分子模式.在 HLA-DR 组合下,核DNA 核DNA是指核中的DNA.这就是PAMP.病原体相关的分子模式败血症这是一种败血症.nDNADNAnDNA是什么意思

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