来自巴勒斯坦加沙地带一家儿科医院的第一份关于碳烯编码多药耐药格拉姆阴性细菌的报告
Nabil Abdullah El Aila1, Nahed Ali Al Laham2, Swapnil Prakash Doijad3,4
1Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Al-Aqsa University, Gaza, Palestine. na.elaila@alaqsa.edu.ps.
BMC microbiology
|October 8, 2024
概括
耐多种药物格拉姆阴性细菌 (GNB) 是一个主要问题. 这项研究发现加沙一家儿科医院的扩展谱β-乳糖酶 (ESBL) 产生GNB的高患病率,检测到显著的抗生素耐药性基因.
科学领域:
- 微生物学与传染病的研究
- 基因组学和分子流行病学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 格拉姆阴性细菌 (GNB) 中的多药耐药性 (MDR) 的全球上升,特别是那些产生扩展光谱β-乳糖酶 (ESBLs) 和卡巴酶的细菌,带来了严重的公共卫生和临床挑战.
- 了解GNB耐药性的遗传基础对于开发有效的治疗策略和感染控制措施至关重要.
研究的目的:
- 从加沙的一家儿科医院分离的ESBL产生格兰阴性菌根 (GNB) 的特征.
- 利用全基因组测序 (WGS) 对这些耐药GNB菌株进行详细的基因组分析.
主要方法:
- 从加沙的纳赛尔儿科医院收集了158个GNB临床隔离物.
- 通过使用双盘协同测试和通过Kirby Bauer方法进行抗生素敏感性概况识别ESBL生产.
- 在15个表型MDR分离物种上进行全基因组测序 (WGS),以确定物种身份和抗生素耐药性基因配置文件.
主要成果:
- 在158个GNB分离物中,超过一半 (58.9%) 是ESBL阳性,其中大肠杆菌和Klebsiella pneumoniae是最常见的物种.
- 在尿液 (64%) 和唾液 (63.6%) 中检测到ESBL生产者的显著流行率. ,伊米和美罗对ESBL生产者表现出最高的有效性.
- 测序的分离物中平均含有6个抗菌素耐药性 (AMR) 基因,其中一些基因含有13个. 值得注意的是,发现了抗卡巴胺的基因 (blaKPC-2,blaPDC-36/12,blaPOM-1).
结论:
- 加沙一家儿科医院中ESBL产生GNB的高患病率强调了MDR带来的重大挑战.
- 鉴定了各种抗生素耐药性基因,包括ESBL和卡巴胺基因的基因,突显了迫切需要有效的抗生素策略.
- 进一步的临床和分子流行病学研究对于了解巴勒斯坦这些耐药GNB菌株相关的风险因素,传播和结果至关重要.
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