甲基斯蒙酸对Lactuca serriola L.的影响:抗氧化防御和生物活性化合物的变化
Maisa Asheri1, Alireza Farokhzad2, Mohammad Reza Naghavi3
1Department of Horticultural Science, Faculty of Agriculture, Urmia University, Iran. maisa.asheri@gmail.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|October 9, 2024
概括
甲基斯酸盐 (MeJA) 叶子喷雾剂显著提高了Prickly中的抗氧化酶活性和含量. 虽然抗氧化剂活性下降,但MeJA显示出增强营养价值的潜力.
科学领域:
- 植物科学 植物科学
- 生物化学 生物化学
- 营养科学 营养科学
背景情况:
- 菜 (Lactuca serriola L.) 是种植菜的野生亲属.
- 了解植物对外源化合物的反应,可以揭示改善作物质量的策略.
- 甲基斯酸盐 (MeJA) 是一种已知的诱导防御反应的植物激素.
研究的目的:
- 为了研究甲基斯酸盐 (MeJA) 对抗氧化酶活性,和黄含量以及 Prickly 中的抗氧化能力的影响.
- 为了确定MeJA治疗对这些参数的剂量依赖性和时间效应.
- 评估MeJA提高Lactuca serriola的营养价值的潜力.
主要方法:
- 在三种MeJA度 (0,200,400μM) 的叶子应用在菜植物上.
- 在处理后的四个不同时间间隔收集植物样本.
- 酶测定超氧化脱氧化酶 (SOD),酶 (CAT),亚斯科巴特过氧化酶 (APX) 和瓜亚科尔过氧化酶 (GPX).
- 总和黄含量的量测量.
- 使用半最大抑制度 (IC50) 试验测量抗氧化剂活性.
主要成果:
- 根据剂量和时间,MeJA治疗显著增加了SOD,CAT,APX和GPX的活性.
- 总和黄含量随着MeJA的应用而增加,在400μMMeJA观察到的最高水平.
- 与对照组相比,MeJA处理的植物的抗氧化活性 (IC50) 较低.
- 峰值的醇含量达到17.02毫克的GAL/g DW,峰值的黄胺含量达到8.3毫克的QUE/g DW.
结论:
- 甲基斯酸盐有效地增强了抗氧化酶活性和酸盐在菜中的积累.
- 虽然整体抗氧化能力 (IC50) 降低,但特定酶和的增加表明复杂的反应.
- 在特定的应用条件下,MeJA治疗有可能改善Lactuca serriola的营养状况.
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