定量实时PCR用于区分Pneumocystis jirovecii感染/入院患者的殖民
Faezeh Rouhi1, Mahzad Erami2, Sepide Rastgufar3
1Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Frontiers in cellular and infection microbiology
|October 9, 2024
概括
实时PCR有效地识别了呼吸道样本中的Pneumocystis jirovecii,使用循环值区分感染和殖民. 这种诊断工具有助于在免疫功能低下的患者中治疗肺囊炎肺炎.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 菌类学 菌类学是指菌类学.
- 传染性疾病 传染性疾病
背景情况:
- 在呼吸道标本中准确识别Pneumocystis jirovecii是具有挑战性的,特别是区分殖民和活跃感染.
- 患有潜在疾病的患者,包括COVID-19和肺衰竭,特别容易患有肺囊性肺炎 (PCP).
- 基于探针的实时PCR (qPCR) 为检测P. jirovecii提供了一个潜在的诊断解决方案.
研究的目的:
- 评估基于探针的实时PCR (qPCR) 诊断有效性,以识别Pneumocystis jirovecii.
- 评估各种呼吸道疾病患者的qPCR性能,包括COVID-19和肺功能不全患者.
- 建立周期值 (Ct) 值范围,以区分P. jirovecii感染与殖民.
主要方法:
- 来自281名呼吸道疾病患者的临床样本使用DNA提取,嵌套PCR和基于探针的qPCR进行了分析.
- 一组112名疑似感染P. jirovecii和肺衰竭的患者进行了详细分析.
- 线粒体大亚单元 (mtLSU) -rRNA基因是PCR方法中的两种放大目标.
主要成果:
- 在281名患者中,P. jirovecii被通过嵌套PCR和qPCR检测到在40名患者中,但差异很小.
- 基于探针的qPCR显示出高诊断性能,与嵌套PCR相比,具有92.5%的灵敏度和98.7%的特异性.
- 建议使用循环值 (Ct) 来区分感染 (≤32),殖民 (>35) 和中间灰色区域 (32 < X ≤ 35).
结论:
- 从qPCR获得的CT值可以有助于区分Pneumocystis肺炎与免疫受损个体的殖民.
- 建议将qPCR结果与临床参数和生物标志物相结合,以提高与肺囊有关的疾病的诊断精度.
- 基于探针的qPCR是检测P. jirovecii的一个有价值的工具,特别是在肺功能衰竭和疑似PCP的患者中.
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