在Lentinula edodes的棕色化过程中,核和线粒体之间的转录体通信
Xiaoxia Song1, Meiyan Zhang1, Mingjie Chen1
1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Journal of agricultural and food chemistry
|October 9, 2024
概括
细胞质置换通过改变核和线粒体基因表达来影响的色. 这项研究揭示了由细胞质源和培养时间影响的内部抗氧化防御和外部黑色素生产机制.
科学领域:
- * 菌群学 * 菌群学
- * 分子生物学 * 分子生物学
- * 植物病理学 植物病理学
背景情况:
- *Lentinula edodes* (石竹) 的色是影响质量的重要因素.
- *以前的研究尚未完全阐明细胞质置换对色的影响背后的分子机制.
- *了解这些机制对于改善的种植和储存至关重要.
研究的目的:
- * 为了研究细胞质置换对L. edodes色的影响的分子基础.
- * 确定关键的核 (N) 和线粒体 (M) 基因以及参与色过程的长非编码RNA (lncRNAs).
- * 探索核和线粒体基因组在调节色特征中的相互作用.
主要方法:
- *核和线粒体mRNAs和lncRNAs的转录组分析.
- * 基因表达特征的比较L808和两个混合体 (L808-A2,L808-B) 在不同的培养时间 (80,100,120天).
- *分析与氧化应激,抗氧化机制和黑色素生产相关的基因表达变化.
主要成果:
- *细胞质源和培养时间显著改变了N,M基因和lncRNA表达.
- *细胞质置换调节的基因参与内部色 (NADPH介导的抗氧化防御对反应性氧物种) 和外部色 (黑色素聚合).
- * 确定了与血重塑,细胞外酶,小分子和NADPH代谢相关的基因;通过M-rps3.3报告了新的核-线粒体通信.
结论:
- *细胞质置换显著影响L. edodes通过改变基因表达的色.
- * 内部色机制涉及NADPH依赖的抗氧化剂保护,防止光引起的氧化应激.
- * 外部棕色的特点是黑色素的积累,受调节膜,酶和新陈代谢的基因的影响;M-rps3介导核-线粒体通信.
更多相关视频
07:26Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
6.1K
14:44Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
Published on: March 14, 2014
12.9K
相关概念视频
Translocation of Proteins into the Mitochondria
3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K
Nuclear Export of mRNA
4.5K
4.5K
Export of Mitochondrial and Chloroplast Genes
3.6K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.6K
The Nucleolus
3.8K
3.8K
Mitochondria
11.3K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.3K
The Inner Mitochondrial Membrane
3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K
