人类尿液在转录基因组和代谢基因组的解卷
Sevahn K Vorperian1,2, Brian C DeFelice3, Joseph A Buonomo2,4
1Department of Chemical Engineering, Stanford University, Stanford, CA, United States.
Clinical chemistry
|October 9, 2024
概括
尿转录组分析提供了一种非侵入性方法,用于检测难以进行切除的组织中的细胞类型变化. 这种方法为早期疾病检测提供了细胞分辨率,并揭示了代谢途径的洞察力.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 由于目前的非侵入性分析中缺乏细胞分辨率,早期检测生殖尿路疾病具有挑战性.
- 现有的以尿为基础的测试往往缺乏用于准确的细胞类型识别所需的全面的转录组数据.
- 尿液中的信使RNA (mRNA) 具有早期疾病检测的潜力,但目前的方法仅限于单基因测量.
研究的目的:
- 调查尿液无细胞RNA (cfRNA) 和沉积物RNA的全转录组分析对非侵入性疾病检测的有用性.
- 为了确定尿液转录组解卷是否可以识别来自生殖尿路和其他组织的细胞类型贡献.
- 为了将尿转录组数据与尿代谢组概况和血cfRNA相关联.
主要方法:
- 从人类尿样中分离和测序cfRNA和沉积物RNA.
- 在健康对照组和结石患者中测量尿代谢组.
- 对尿转录组进行细胞类型解的生物信息分析,并与血cfRNA和代谢组数据进行比较.
主要成果:
- 尿液转录基因组解卷确定了来自生殖尿路和其他高周转率固体组织的细胞类型贡献.
- 与血cfRNA的比较显示了丰富的代谢途径和尿液中独特的细胞类型谱.
- 尿液转录和代谢学数据的整合突出了参与氨基酸代谢和近道管功能的代谢途径.
结论:
- 尿液cfRNA和沉积物RNA的非侵入性全转录组测量为难以进行切除的组织提供细胞类型分辨率.
- 尿转录组分析反映了由尿代谢组可测量的代谢途径中丰富的信号.
- 这种方法为早期疾病检测和监测提供了一个有前途的非侵入性液体活检.
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