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下一代测序对于实验室诊断瑞克症的有用性

Fanfan Xing¹, Chaowen Deng¹, Jinyue Huang¹

¹Department of Infectious Diseases and Microbiology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, Guangdong, China.

PLoS neglected tropical diseases

|October 9, 2024

概括

下一代元基因组测序 (mNGS) 有助于诊断困难的病病例. 这种分子工具补充了传统的血清学,用于识别斑点发烧,伤寒组和擦伤伤寒感染.

科学领域:

传染性疾病传染性疾病
分子诊断学分子诊断学
基因组学就是基因组学.

背景情况:

里克塞症包括多种不同的,培养负的感染,带来了诊断挑战.
传统的实验室方法用于诊断病,往往是困难和耗时的.

研究的目的:

评估元基因组下一代测序 (mNGS) 在诊断各种皮病感染中的实用性.
为了将mNGS与常规诊断方法进行比较.

主要方法:

这是一项为期12个月的研究,涉及6名疑似患有病感染的患者.
诊断是在血液,支气管支气管洗,肺活检和血清样本上使用元基因组下一代测序 (mNGS) 进行的.
传统的血清学测试 (Weil-Felix) 和IgM检测也用于比较.

主要成果:

在一个日本斑点热病例中,mNGS迅速发现了Rickettsia japonica.
在两个患有胸部感染的免疫受损患者中检测到Rickettsia felis.
mNGS在灌木伤寒病例中发现了Orientia tsutsugamushi,后来被IgM证实.
mNGS的结果促使对韦尔-费利克斯测试进行了重新评估,揭示了两例伤寒组病例的阳性标位.

结论:

下一代元基因组测序 (mNGS) 是诊断病的宝贵工具,包括斑点性发烧,伤寒和擦伤伤寒.
mNGS可以在各种临床标本中成功检测皮病原体.
mNGS补充了血清学检测,并有助于挑战病的诊断,特别是在培养负的环境中.

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