LlbHLH87与LlSPT相互作用,通过激活中的LlHSFA2和LlleIN3来调节耐热性
Ze Wu1,2,3, Xue Gong1,2, Yinyi Zhang1,2
1Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
The Plant journal : for cell and molecular biology
|October 9, 2024
概括
莉莉莉莉莉莉 莉莉莉 莉莉莉 莉莉莉
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 基本螺旋环螺旋 (bHLH) 蛋白质是植物转录的关键因素.
- 它们在耐热性中的作用基本上尚未被探索.
- 了解热应激反应对农业至关重要.
研究的目的:
- 鉴定和描述一种参与植物耐热的新型bHLH蛋白.
- 阐明LlbHLH87在耐热性中的分子机制.
- 研究与其他调节蛋白的相互作用.
主要方法:
- 在百合花 (Lilium longiflorum) 中基因识别和表达分析.
- 在酵母和植物细胞中进行蛋白质局部化和交换活化测试.
- 基因沉默和过度表达研究在百合花和阿拉比多普西斯.
- 促进体结合测定和蛋白质-蛋白质相互作用研究.
主要成果:
- LlbHLH87是一种可热诱导的bHLH蛋白质,增强了植物的耐热性.
- LlbHLH87直接激活了 LlHSFA2 和 LlEIN3 的基因表达.
- LlSPT蛋白质对抗LlbHLH87的功能,调节热应激反应.
- 同时表达的LlbHLH87和LlSPT在短暂和长时间的热下不同地影响热耐受性.
结论:
- LlbHLH87是百合花热耐受性的关键调节剂.
- LlbHLH87通过激活与热应激相关的关键基因而起作用.
- 与LlSPT的相互作用为热适应提供了一个复杂的调节机制.
相关概念视频
02:45Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
01:29Translational Regulation
1
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
1
02:27Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K
01:25Inducible Operons: lac Operon
9
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
9
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K