基于多种细菌的直接养微生物减轻了沙门氏菌的入侵,并支持体外表皮质完整性
Raphaele Gresse1, Bruno I Cappellozza1, Lena C Capern1
1Novonesis, Planetary Health & Biosolutions, Hørsholm 2970, Denmark.
Journal of animal science
|October 9, 2024
概括
一种新的直接养微生物 (DFM) 显著减少了沙门氏菌Typhimurium的入侵,并在体外证明了对沙门氏菌Dublin的对抗作用. 这种DFM还支持肠道细胞完整性,抵御病原体和氧化挑战,对牲畜健康有很大的前景.
科学领域:
- 微生物学和动物科学
- 胃肠道健康与免疫学
背景情况:
- 细菌感染,特别是Salmonella enterica serovars Typhimurium和Dublin,对牲畜的健康,表现和食品安全构成重大威胁.
- 直接养微生物 (DFM) 已被认为具有支持胃肠功能和减轻细菌病原体不良影响的潜力.
研究的目的:
- 评估一种新的多种类细菌基DFM在减少沙门氏菌Typhimurium入侵及其对沙门氏菌的对抗活性中的有效性.
- 评估DFM在病原体和氧化应激条件下保持肠上皮细胞完整性的能力.
主要方法:
- 在体外共化模型使用HT29-MTX-E12细胞来评估S. Typhimurium入侵减少.
- 阿加尔井扩散试验以评估DFM对S.都柏林的对抗作用.
- 用Caco-2细胞测量体电阻 (TEER) 测定,以测量在各种DFM剂量的挑战条件下 (S. Typhimurium或过氧化) 的肠道上皮细胞完整性.
主要成果:
- 在HT29-MTX-E12细胞中,DFM显著降低了S. Typhimurium入侵率90.8%,细菌活力降低了21.0%.
- 一个明显的抑制区 (8.6毫米) 证实了DFM对S.都柏林的对抗性.
- 在所有测试剂量中,DFM支持肠道细胞完整性免受S. Typhimurium挑战,并在最高剂量时免受过氧化挑战.
结论:
- 经过测试的DFM显示出作为减轻反动物中Salmonella enterica感染的策略的巨大潜力.
- 这些体外检验结果支持DFM在改善动物健康,食品安全和公共卫生方面的作用.
- 需要进一步的体内研究来验证这些有希望的初步结果.
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