通过使用二极管阵列检测,充电气溶检测和质谱仪的HILIC方法分离聚醇:适用于富含stilbenoids的葡萄藤提取物
Karen Gaudin1, Josep Valls-Fonayet2, Rémy Cordazzo1
1Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, F-33140 Villenave d'Ornon, France.
Journal of chromatography. A
|October 9, 2024
概括
这项研究探索了水友互动液体染色学 (HILIC) 用于多分析,发现一种氨基静止相与酸是最佳的. 这种HILIC方法与充电气溶检测 (CAD) 相结合,为分析植物提取物 (如) 提供了有价值的替代方案.
科学领域:
- 分析化学 分析化学
- 植物科学 植物科学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 植物提取物的表征传统上依赖于反相液态色谱 (RP).
- 水友互动液体染色学 (HILIC) 呈现了对多分析的直角方法.
- 评估各种HILIC静止阶段对于优化多保留至关重要.
研究的目的:
- 评估五个HILIC静止相对31个多醇标准的保留能力.
- 调查移动相组合和检测方法对多分离的影响.
- 为了建立最佳的HILIC条件来分析富含stilbenoid的植物提取物.
主要方法:
- 使用31种多标准对五种HILIC静止相 (裸,乙烯桥,胺基,氨基,基) 的选.
- 评估了三种检测方法:二极管阵列探测器 (DAD),带电气溶探测器 (CAD) 和质谱 (MS).
- 优化移动相组合,包括pH调节剂 (TFA,酸) 和缓冲剂 (氨).
主要成果:
- 基于的支物表现出较差的聚烯保留,除了酸.
- 对于化合物的静止阶段有效性按以下顺序增加:zwitterionic < amide < amino.
- 基于酸的移动相的氨基静止相为多提供了最佳的HILIC条件.
- 希利克-CAD组合被证明是有效的分析 stilbenes,与聚合度的化顺序相关联.
结论:
- 特别是在氨基基静止阶段和酸移动阶段的HILIC对于聚醇分析是有效的.
- HILIC-CAD组合为RP提供了一种补充方法,用于分析植物提取物中的 stilbenes.
- 在优化的HILIC条件下,成功分析了富含stilbenoid的葡萄藤提取物,证明了其实际应用.
更多相关视频
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
3.8K
10:30A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
6.8K
相关概念视频
01:21Mass Spectrometry: Complex Analysis
732
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
732
01:11High-Performance Liquid Chromatography: Introduction
1.6K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
1.6K
01:15High-Performance Liquid Chromatography: Types of Detectors
496
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
496
01:14Gas Chromatography–Mass Spectrometry (GC–MS)
4.0K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
4.0K
00:57High-Performance Liquid Chromatography: Instrumentation
1.4K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
1.4K
01:19Gas Chromatography: Types of Detectors-II
338
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
338