在死后储存期间,Esox Lucius的肉质的变化:基于溶解体-线粒体的亡途径
Hui Li1, Xuemei Fan1, Xin Guo1
1Key Laboratory of Characteristics Agricultural Product Processing and Quality Control (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China; School of Food Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China.
储存期间鱼类的软化与 lysosome-mitochondrial apoptosis 途径有关. 费里丁降解和溶酶体铁变化触发了这个过程,激活了Esox Lucius中的细胞死亡机制.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 食品科学 食品科学 食品科学
背景情况:
- 鱼死后软化会影响质地和质量.
- 溶解体和线粒体通路参与细胞亡.
研究的目的:
- 为了研究 lysosome-mitochondrial apoptosis 途径在鱼软化中的作用.
- 在储存期间探索ferritin降解,溶酶体铁和Esox Lucius中亡之间的关系.
主要方法:
- 斯皮尔曼的相关性分析.
- 传输电子显微镜 (TEM) 的使用
- 道染色 道染色
主要成果:
- 观察到费里丁水平降低和溶酶体铁含量变化.
- lysosomal 膜的不稳定性与 lysosomal 铁和ferritin 降解的增加相关.
- 证实了酶级联 (酶-9/-3) 的激活,以及对亡的形态证据.
结论:
- lysosome-mitochondrial apoptosis 途径在死后存储期间在Esox Lucius中是活跃的. 在死后存储期间, lysosome-mitochondrial apoptosis 途径是活跃的.
- 费里丁降解和溶解体铁的增加是溶解体损伤的关键发起者.
- lysosomes 释放的 cathepsin D 是线粒体亡的一个关键环节.
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